花椰菜温敏雄性不育系GS-19花蕾发育过程中内源激素的动态变化分析

为了探究内源激素在花椰菜温敏雄性不育系GS-19育性调控中的作用,以花椰菜温敏雄性不育系GS-19为材料,采用高效液相色谱法,对花椰菜温敏雄性不育系的不育株和可育株花蕾不同发育时期及叶片中赤霉素(GA)、生长素(IAA)、脱落酸(ABA)及玉米素核苷(ZR)的动态变化进行比较分析。结果表明,花椰菜温敏雄性不育系花蕾和叶片发育过程中,不育株和可育株内源激素GA、IAA、ABA和ZR的变化均存在明显差异。不育株花蕾中的GA和ABA含量总体呈上升趋势,GA含量在造孢时期、四分体时期及花粉成熟期的含量均显著高于可育株,分别为281.54、355.37和350.13μg·g~(-1),比可育株花蕾高45.1%、210.4%和54.5%,而ABA含量只在四分体时期和花粉成熟期显著高于可育株,分别为2.22μg·g~(-1)和2.88μg·g~(-1),比可育株花蕾高82%和35.2%;不育株花蕾中的IAA含量先降后升,在造孢时期和花粉成熟期显著高于可育株,分别为1.97μg·g~(-1)和2.55μg·g~(-1),比可育株花蕾高52.7%和82.1%,但不育株花蕾的ZR含量先升后降,在四分体和花粉成熟期显著高于可育株,分别为320μg·g~(-1)和170μg·g~(-1),比可育株花蕾高580.9%和243.9%。不育株的IAA/ABA呈"V"字型变化,在四分体时期比值最低,而GA/ABA和ZR/ABA呈倒"V"字型的变化,在四分体时期比值最高。叶片中ABA和ZR未检出,GA和IAA含量不育株显著高于可育株。说明内源激素含量升高和多种激素间的平衡关系被打破,比例失调,阻碍了小孢子的正常发育,导致花椰菜温敏雄性不育系GS-19花粉败育。本研究结果对揭示内源激素在雄性败育过程中可能存在的作用机制具有重要意义,为进一步揭示花椰菜温敏雄性不育的遗传机制积累了资料。

Dynamic Analysis of Endogenous Hormones during Bud Development Stage in Cauliflower Thermo-sensitive Male Sterile Line GS-19

In order to investigate effect of endogenous hormones on fertility control of the thermo-sensitive male sterile line GS-19 in cauliflower,taking GS-19 as the material,high performance liquid chromatography (HPLC) was adopted to comparatively analyze the dynamic changes of gibberellin (GA),auxin (IAA),abscisic acid (ABA) and zeatin riboside(ZR) of the leaves different development stages buds in sterile and fertile plantsat.The results showed that the changes of endogenous hormone (GA,IAA,ABA and ZP) of sterile and fertile plants had obvious differences in leaves and different developmental stages buds.GA and ABA content of buds of sterile plants appeared upward trend in general,and GA content was significantly higher than that of fertile plants at sporogenous stage,tetrad stage and pollen maturation stage.They were 281.54,355.37 and 350.13 μg·g-1 respectively,which was 45.1％,210.4％ and 54.5 ％ higher than the fertile plants,while ABA content was higher than the fertile plants only in tetrad stage and pollen maturation stage,was 2.22 μg·g-1and 2.88 μg·g-1,which was 82％ and 35.2％ higher than the fertile plants.IAA content of buds of sterile plants decreased at first and then increased,which was significantly higher than the fertile plants in sporogenous stage and pollen maturation stage,was 1.97 μg·g-1 and 2.55 μg·g-1,which was 52.7％ and 82.1％ higher than the fertile plants respectively.But ZR content rose at first and then decreased,which was significantly higher than the fertile plants in tetrad stage and pollen maturation stage,was 320 μg·g-1 and 170 μg·g-1,which was 580.9％ and 243.9％ higher than the fertile plants.Compared with fertile plants,IAA/ABA of the sterile plants buds appeared a V type change,which was minimum in tetrad stage.While GA/ABA and ZR/ABA of which appeared an inverted V change,which was maximum in tetrad stage.ABA and ZR were not detected in leaves and GA and ABA content of the sterile plants were significantly higher than the fertile plants.The results showed that the rise of endogenous hormones and the break of balance on many hormones hindered the normal development of microspore,which led to the pollen abortion of GS-19.The investigation was very important in revealing the relationship between endogenous hormones and male abortion,and provided much important information for revealing the genetic mechanism of the cauliflower thermo-sensitive male sterility.