胶膜菌属真菌与兰科植物的共生体系建立

为建立兰科植物与菌根真菌的共生体系,本研究筛选出适宜的共生培养基,采用ITS-rDNA分析鉴定了6株菌根真菌,并将6株菌与兰科植物共生培养,利用台盼蓝染色法检验共生体是否建立。结果表明,2.5 g·L~(-1)燕麦琼脂培养基为树兰和胶膜菌属真菌共生的最适宜培养基,供试兰科植物中仅树兰可与6株胶膜菌属真菌共生,其中一株美孢胶膜菌(Tco 2)促进树兰种子萌发和原球茎发育的效果最好。本研究筛选出可促进树兰高效萌发成苗的共生真菌和培养基,为真菌与植物共生关系研究提供了良好的试验系统,为树兰的生产繁殖奠定了基础,对保护兰科植物物种资源具有重要意义。

Symbiosis Established Between Orchid and Tulasnella spp.Fungi

In this study,the ITS-rDNA sequence analysis was used to identify six strains of mycorrhizal fungi,then coculture them with orchids,and the trypan blue staining test was used to verify the symbiosis,in order to establish the symbiosis between orchid and mycorrhiza,and also to screen a suitable medium for symbiotic culturing.The results showed that all Tulasnella spp.strains in this study can establish symbiosis with Epidendrum secundum,and 2.5 g· L-1 oatmeal agar medium was the most suitable co-culture medium for them,a strain effectively promoted the seed germination and protocorm differentiation of Epidendrum secundum was Tulasnella calospora (Tco2).The mycorrhizal fungi and co-culture medium screened for promoting the seed germination of Epidendrum secundum provide the fungi and plant symbiotic relationship research a good experimental system model,which will play a foundation for reproducing and it would be of great significance on protection of species resource of Epidendrum secundum.