Methods to overcome seed dormancy in Echinacea angustifolia DC |
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| Institution: | 1. Key Laboratory of Thermo-Fluid Science and Engineering, Ministry of Education, School of Energy and Power Engineering, Xi’an Jiaotong University, Xi’an, Shaanxi 710049, China;2. Donghai JA Solar Technology Co., Ltd., Lianyungang, Jiangsu 222300, China;1. Department of Chemistry – “Sapienza”, University of Rome, Italy;2. Department of Chemistry and Chemical Technologies – University of Calabria, Italy;1. Department of Biology, University of Florence, Italy;2. Department of Biology, University of Pisa, Italy;1. Graduate School of Agricultural Science, Kobe University, Rokkodai 1-1, Nada-ku, Kobe 657-8501, Japan;2. Departmento de Biotechnología, Facultad de Ciencias Químicas, Universidad Nacional de Asunción, San Lorenzo, Paraguay;3. Graduate School of Agriculture, Kyoto University, Kitashirawaka-Oiwake, Sakyo-ku, Kyoto 606-8502, Japan;1. Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200438, China;2. Jiangxi Province Key Laboratory of Watershed Ecosystem Change and Biodiversity, Center for Watershed Ecology, Institute of Life Science and School of Life Sciences, Nanchang University, Nanchang 330031, China |
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| Abstract: | Some factors (light, prechilling, gibberellic acid, ethylene) affecting germination of seeds of Echinacea angustifolia DC. were investigated. Without any pretreatment, the seeds germinated better in darkness than in light, however, percentages germination were low in both cases. GA3 did not increase germination in light. Prechilling for 7–15 days in light or in darkness hardly affected percentage germination but significantly increased the rate of germination. Ethephon during prechilling resulted in a large increase of percentage and rate of germination in light, but had hardly any effect on germination in darkness. The results showed that a prechilling treatment for about 11 days at 5°C in a 1 mM ethephon solution in continuous light, followed by a 2-week germination period in light (24 h per day) at 20/30°C, can induce >90% seed germination in E. angustifolia. The prechilling treatment in ethephon also increased the rate of germination. |
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