Suitability of isozyme,RAPD and AFLP markers to assess genetic differences and relatedness among fig (Ficus carica L.) clones |
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| Affiliation: | 1. Durham School of Architectural Engineering and Construction, University of Nebraska – Lincoln, 1110 S. 67th Street, Omaha, NE 68182, USA;2. School of Mechanical Engineering, Purdue University, 585 Purdue Mall, West Lafayette, IN 47907, USA;1. Instituto de Ciências Exatas e Naturais, Universidade Federal de Mato Grosso (UFMT), Rondonópolis, MT, Brazil;2. Departamento de Farmácia, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, MG, Brazil;3. Instituto Nacional de Ciência e Tecnologia em Bioanalítica, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil;4. Laboratório de Parasitologia, Universidade Federal de Juiz de Fora (UFJF), Campus Governador Valadares, MG, Brazil;5. Laboratório de Doenças de Chagas, Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Universidade Federal de Ouro Preto (UFOP), Ouro Preto, MG, Brazil;6. Departamento de Química, Universidade Federal do Maranhão (UFMA), São Luís, MA, Brazil;7. Laboratório de Biomarcadores de Diagnóstico e Monitoração, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz (CPqRR-FIOCRUZ/MG), Belo Horizonte, MG, Brazil;1. Universidade Estadual de Santa Cruz (UESC), Brazil;2. Animal Science Graduate Program, Universidade Estadual de Santa Cruz (UESC), Brazil;3. Center for Computational Biology and Biotechnology Information Management (NBCGIB), Universidade Estadual de Santa Cruz (UESC), Brazil |
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| Abstract: | Sarilop is the main and standard cultivar for commercial dried fig (Ficus carica L.) production in Turkey. Eleven of the most promising Sarilop clones and one clone of Sarizeybek, all selected from a former agronomic evaluation, were analysed by three molecular marker techniques, isozymes, RAPDs and AFLPs. The resolution power and the accuracy of these three analytical techniques, in distinguishing among fig clones, were determined. The analysis of five isozyme systems permitted the discrimination between the two cultivars, Sarilop and Sarizeybek. Besides the discrimination between the two fig cultivars, the use of 31 10-mer primers in RAPD analysis allowed splitting the 11 Sarilop clones into two groups of genetic similarity, but not to distinguish between all the clones. The AFLP™ technology showed a much higher multiplex ratio than the RAPD technique (42.4 vs. 6.1) and eight combinations of EcoRI/MseI primers were enough to clearly distinguish between all the Sarilop clones. |
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