An investigation on the effect of different plant growth regulating compounds in in vitro shoot tip and node culture of lemon seedlings |
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| Affiliation: | 1. Faculty of Physical Chemistry, University of Belgrade, Studentski trg 12-16, P.O. Box 473, 11001 Belgrade, Serbia;2. Institute of Physics, University of Belgrade, Pregrevica 118, Zemun, Serbia;1. Department of Chemistry, University of Patras, GR-26504 Patras, Greece;2. Department of Food Technology, TEI of Peloponnese, 24100 Kalamata, Greece;1. Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford OX1 3QZ, United Kingdom;2. Micro-/Nano-Electrochemistry – Center for Electrochemical Sciences (CES), Faculty of Chemistry and Biochemistry, Ruhr-University Bochum, D-44780 Bochum, Germany;1. Department of Chemistry, “Sapienza” University of Rome, p.le A.Moro 5, 00185 Rome, Italy;2. Department of Chemistry and Chemical Technologies, University of Calabria, via P.Bucci, 87036 Arcavacata di Rende, Italy;1. ICAR-Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata 700120, India;2. Sunnhemp Research Station, ICAR-Central Research Institute for Jute and Allied Fibres, Pratapgarh, Uttar Pradesh, India;1. Department Environmental Physics and Irrigation, Institute of Soil, Water and Environmental Sciences, ARO Volcani Center, Israel;2. Department of Soil Chemistry, Plant Nutrition and Microbiology, Institute of Soil, Water and Environmental Sciences, ARO Volcani Center, Israel;3. Department Horticultural Sciences, Institute of Plant Sciences, ARO Gilat Research Station, Israel;4. Department of Soil and Water Sciences, The Robert H. Smith Faculty of Agriculture, Hebrew University of Jerusalem, Israel |
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| Abstract: | The possible application of some less commonly used in vitro growth regulating compounds is outlined. A number of treatments were applied to determine the best way of inducing in vitro shoot proliferation and rooting on a modified Driver–Kuniyuki [HortScience 19 (1984) 507] basal medium of lemon (Citrus limon (L.) Burm, f. cv. Interdonato) seedlings. 6-Benzyladenine (BA) alone (1, 2 and 4 mg l−1) and in combination with either orange juice (10%, v/v), silver nitrate (3 mg l−1), gibberellic acid (GA3) (0.1 mg l−1 at the establishment stage and 0.5 mg l−1 at all combinations during the proliferation stage) or abscisic acid (ABA) (0.2 mg l−1 only at the establishment stage) were used to stimulate shoot formation during the establishment and the proliferation stage. The combination of BA with ABA gave a high rate of shoot formation, while GA3 and silver nitrate enhanced shoot elongation. When these shoots were transferred to the rooting stage, the effect of application of two different auxins (indole-3-butyric acid (IBA) and α-napthaleneacetic acid) was examined, as well as different methods of application (auxin added to the basal medium and auxin application by dipping the base of the explant in auxin solution). Dipping the base of the explants in a 50% ethanol solution of IBA at 1000 mg l−1 for 5 s resulted in 80% rooting with subsequent 90% survival of these explants, during acclimatization under mist. |
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