Morphological and physiological characteristics of micropropagated strawberry plants rooted in vitro or ex vitro |
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| Institution: | 1. Laboratoire de chimie de la matière condensée de Paris (LCMCP), collège de France, Sorbonne universités, UPMC université Paris 06, UMR CNRS 7574, 11, place Marcelin-Berthelot, 75005 Paris, France;2. Laboratoire de physique des solides, université Paris XI, 91405 Orsay cedex, France;3. Service d’explorations fonctionnelles, hôpital Tenon, AP–HP, 4, rue de la Chine, 75970 Paris cedex 20, France;4. Inserm, UMRS 1155, UPMC, hôpital Tenon, 75970 Paris, France;5. Service d’urologie, hôpital St-Louis, 1, avenue C.-Vellefaux, 75010 Paris, France;1. Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Lodz, Poland;2. Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Warsaw, Poland;1. Department of Biotechnology, Osmania University, Hyderabad, Telangana 500 007, India;2. Biotechnology Unit, Kanchi Mamunivar Government Institute for Postgraduate Studies and Research, Puducherry 605 008, India;3. Department of Botany, Siddha Clinical Research Unit, Central Council for Research in Siddha, Palayamkottai, Tamil Nadu 627 002, India;4. Department of Biotechnology, Kakatiya University, Warangal, Telangana 506 009, India;5. Department of Life Sciences, Presidency University, College Street, Kolkata 700 073, India;6. Department of Botany & Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia;7. Agricultural Research Station, Fort Valley State University, Fort Valley, GA 31088, USA;8. Department of Botany, Osmania University, Hyderabad, Telangana 500 007, India;1. Department of Genetics and Horticultural Plant Breeding, Institute of Plant Genetics, Breeding and Biotechnology, Faculty of Agrobioengineering, University of Life Sciences in Lublin, Akademicka 15 Street, 20-950 Lublin, Poland;2. Department of Chemistry, Faculty of Food Science and Biotechnology, University of Life Sciences in Lublin, Akademicka 15 Street, 20-950 Lublin, Poland;1. College of Horticulture, Shenyang Agricultural University, 120 Dongling Road, Shenyang, 110866, China;2. Institute of Carbon Materials Science, Shanxi Datong University, Datong, Shanxi, 037009, China;3. Liaoning Key Laboratory of Strawberry Breeding and Cultivation, Shenyang Agricultural University, 120 Dongling Road, Shenyang, 110866, China |
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| Abstract: | In the classical method of strawberry micropropagation, the rooting phase is done in vitro. The trials were undertaken to replace in vitro rhizogenesis by a direct ex vitro rooting. The aim of the present study was to evaluate the morphological and physiological status of strawberry plants rooted by both methods. The micropropagated shoots of strawberry, cultivars Senga Sengana, Kent and Kama were rooted by the standard method at the in vitro stage or they were treated as soft cuttings and rooted ex vitro (in non-sterile conditions). After a 4-week rooting period the plantlets rooted ex vitro had larger root systems than in vitro-rooted ones, as evidenced by a significantly lower ratio of shoot to root dry weights (2.95, 3.33 and 4.24, respectively, for ex vitro rooted Senga Sengana, Kent and Kama plants versus 5.09, 6.95 and 5.04 for in vitro rooted plants of the same cultivars). During subsequent growth, differences in development increased and were most pronounced in runner formation, more than twice as many runners were formed by ex vitro, than by in vitro-rooted plants. Chlorophyll fluorescence parameters showed that photochemical activity was similar in the leaves from plants rooted in vitro and ex vitro. Values of chlorophyll fluorescence parameters indicated that persistent leaves play the key photosynthetic role at the beginning of the growth period. With the formation of new leaves, the photosynthetic activity of the persistent leaves decreased and their function was taken over by the newly formed ones. |
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