盐胁迫对NHC牧草IAA、ABA、CaM及NADKase的动态变化

研究NewHy Crested Wheatgrass牧草(NHC)盐胁迫下部分胞间胞内信号分子的变化及部分耐盐机理，将为改良辽宁营口沿海产业基地NaCl型盐碱地奠定基础。以Hoagland培养液培养NHC牧草，3周后对幼苗进行NaCl溶液和土壤盐溶液胁迫处理，并测定吲哚乙酸(IAA)、脱落酸(ABA)、钙调蛋白(CaM)及NAD激酶(NADKase)活性。结果表明：在100、200 mmol/L NaCl盐及土壤盐处理条件下，IAA含量无差异，但比对照极显著升高；其他各生理指标均随浓度升高而极显著升高。由此可见,NHC牧草通过ABA和IAA含量的变化，激活钙信号系统，增加CaM含量，激活NADKase活性，调节细胞代谢活力，从而提高耐盐胁迫能力。NHC牧草耐盐临界浓度分别为土壤盐浓度200 mmol/L、NaCl浓度 300 mmol/L。同样浓度条件下，土壤盐溶液对NHC牧草的伤害比NaCl溶液更大。

Dynamic variation of IAA, ABA, CaM and NADase in NewHy Crested Wheatgrass under salt stress

The partial salt tolerance mechanism and partial dynamic variation of intercellular and intracellular signals of NewHy Crested Wheatgrass(NHC)were studied for improving the saline-alkali soil that mainly contains NaCl in Panjin City of Liaoning Province.The seedlings of NHC were cultured with Hoagland nutrient solution,and then,the seedlings were treated with soil salt solution and NaCl salt solution after 3 weeks.The content of IAA,ABA and CaM and the activity of NADKase were tested after treatment.The results indicated that only the content of IAA did not significantly change in 100,200 mmol/L of soil salt solution and NaCl salt solution but it was different with control;while other physiological indexes increased dramatically with the concentration of salt solution.It could be concluded that NHC activates the Ca2 signal system through the change of ABA and IAA and increases the content of CaM,then activates NADKase,adjusts cells metabolic activity,so that the tolerance to salt stress could be increased.The threshold concentrations for NHC were 300 mmol/L NaCl salt solution or 200 mmol/L of soil salt solution.The damage of soil salt solution on NHC was greater than NaCl solution under the same concentration.