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| 发情牛血清在猪卵母细胞成熟中的应用 | |
| 引用本文： | 李秋艳,朱庆鸿,卫恒习,李燕,郭英,戴蕴平,李宁.发情牛血清在猪卵母细胞成熟中的应用[J].农业生物技术学报,2010,18(1):192-197. |
| 作者姓名： | 李秋艳 朱庆鸿 卫恒习 李燕 郭英 戴蕴平 李宁 |
| 作者单位： | 1. 中国农业大学生物学院,农业生物技术国家重点实验室,北京,100193;北京济普霖生物技术有限公司,北京,100193 2. 中国农业大学生物学院,农业生物技术国家重点实验室,北京,100193 3. 北京济普霖生物技术有限公司,北京,100193 |
| 基金项目： | 国家高技术研究与发展计划项目 |
| 摘 要： | 为了检验发情牛(Bos taurus)血清在猪卵母细胞成熟中的应用,实验分别在成熟培养液中加入不同的血清,观察卵母细胞的成熟效果,并通过孤雌激活胚胎和重构胚的体外发育能力来判断血清对卵母细胞质量的影响:研究还进行了用发情牛血清成熟的卵母细胞构建的核移植胚胎的体内发育试验.结果表明,发情牛血清,成年牛血清和胎牛血清在猪卵母细胞的成熟率方面没有显著差异(P>0.05);卵母细胞成熟时加入不同的血清,对卵母细胞体外发育能力的影响不显著(P>0.05);来自含发情牛血清成熟液培养的卵母细胞,与胎儿成纤维细胞构建的克隆胚胎能够完成全期发育. |
| 关 键 词： | 猪 卵母细胞 体外成熟 发情牛血清 孤雌激活 核移植 |
Application of Estrous Cow Serum on Porcine Oocyte Maturation |
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| Li Qiuyan , Zhu Qinghong Wei Hengxi Li Yan Guo Ying Dai Yunping , Li Ning State Key Laboratory for Agrobiotechnology.Application of Estrous Cow Serum on Porcine Oocyte Maturation[J].Journal of Agricultural Biotechnology,2010,18(1):192-197. | |
| Authors: | Li Qiuyan Zhu Qinghong Wei Hengxi Li Yan Guo Ying Dai Yunping Li Ning State Key Laboratory for Agrobiotechnology |
| Institution: | Li Qiuyan 1,2 Zhu Qinghong 1 Wei Hengxi 1 Li Yan 2 Guo Ying 1 Dai Yunping 1,2 Li Ning 1* 1 State Key Laboratory for Agrobiotechnology Department of Animal Physiology,College of Biological Science,China Agricultural University,Beijing,100193,2 Jipulin Biotech Ltd. |
| Abstract: | In order to determine the effect of estrous cow(Bos taurus) serum on porcine oocyte maturation,various sera was added to maturation medium and the matured results of oocyte were observed.The effect of serum on oocyte quality was tested by in vitro developmental abilities of parthenactivation and reconstructed embryo.Moreover,in vivo development ability of nuclear transfer embryos constructed by oocytes matured with medium supplemented estrous cow serum was tested.Results indicated that there was no signific... |
| Keywords: | Porcine Oocyte In vitro maturation Estrous cow serum Parthenactivation Nuclear transfer |
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