气管给药法检测甲醛对小鼠肺组织氧化损伤的研究

为观察由甲醛引起的小鼠肺组织氧化损伤,采用气管给药法将不同剂量的液态甲醛通过气管直接给药,连续染毒处死后,摘取小鼠肺组织,测定肺表面活性物质相关蛋白A（SP-A）含量、肺组织SOD活力以及MDA含量;制作肺组织HE染色切片,观察小鼠肺组织病理变化。结果表明,高、低剂量组的SP-A含量及肺组织SOD活力均显著低于生理盐水对照组（P〈0.05）,高、低剂量组的肺组织MDA含量均显著高于生理盐水对照组（P〈0.05）;光镜下观察可见高、低剂量组的肺泡间质细胞增多,且炎症细胞增多,发生炎性浸润。综上提示,甲醛可引起小鼠肺组织氧化损伤。

Evaluation of Oxidative Damage of Lung Tissue Induced by Formaldehyde Using Intratracheal Medication in Mice

The objective of this study was to observe the oxidative damage of lung tissue induced by formaldehyde in mice. The experimental mice were administrated with multiple doses of liquid formaldehyde with different concentrations by intratracheal medication. Subsequently, the mice were sacrificed, and their lung tissue were collected to determine the pulmonary surfactant-associated protein A （SP-A） content, superoxide dismutase （SOD） activity and malondialdehyde （MDA） content. Furthermore, the lung tissue sections were prepared, and the pathological changes were observed by means of light microscopy. The results showed that the high and low dosage groups had significantly lower SP-A content and SOD activity compared to the saline solution control group （P〈0.05） and had significantly higher MDA content （P〈0.05）; the increased number of stromal cells in pulmonary alveoli and inflammatory cells were observed, and the inflammatory cell infiltration were found. The combined data suggest that formaldehyde is able to cause oxidative damage of lung tissue in mice.