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| 两种不同种源地白榆的组织培养与叶片再生研究 | |
| 引用本文： | 王静华,侯建生,刘桂林,梁海永,于小清. 两种不同种源地白榆的组织培养与叶片再生研究[J]. 中国农学通报, 2009, 25(5): 110-115 |
| 作者姓名： | 王静华 侯建生 刘桂林 梁海永 于小清 |
| 作者单位： | 1. 河北农业大学园林与旅游学院,河北保定,071001 2. 河北政法职业学院园林系,石家庄,050061 3. 河北农业大学林学院,河北保定,071001 4. 河北农业大学园艺学院,河北保定,071001 |
| 摘 要： | 采用河北沧州市和内蒙赤峰市两种不同种源地的白榆茎段作为外殖体进行组织培养，在附加不同浓度激素的培养基上对其进行增殖、叶片再生及生根培养，建立其组织培养及叶片再生体系。结果表明，来自河北沧州市的白榆最适分化培养基是MS培养基+6-BA 0.10㎎/L+IBA0.005㎎/L，最适生根培养基是MS培养基+IBA0.01㎎/L，而来自内蒙赤峰市的白榆最适分化培养基是MS+6-BA 0.10㎎/L+IBA0.025㎎/L，最适生根培养基是MS培养基+IBA0.05㎎/L。两种白榆叶片再生的最适培养基均为MS+TDZ0.005㎎/L+IAA0.005㎎/L. |
| 关 键 词： | 白榆 组织培养 种源地 叶片再生 |
| 收稿时间： | 2008-11-10 |
| 修稿时间： | 2008-11-20 |
Study on tissue culture and leaf regeneration of two kind of different provenances Siberian elm |
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| Wang Jinghu,Hou Jiansheng,Liu Guilin,Liang Haiyong,Yu Xiaoqing. Study on tissue culture and leaf regeneration of two kind of different provenances Siberian elm[J]. Chinese Agricultural Science Bulletin, 2009, 25(5): 110-115 | |
| Authors: | Wang Jinghu Hou Jiansheng Liu Guilin Liang Haiyong Yu Xiaoqing |
| Affiliation: | Wang Jinghua,Hou Jiansheng, Liu Guilin, Liang Haiyong, Yu Xiaoqing (1.College of Landscape Architecture and Tourism, Agricultural University of Hebei, Baoding Hebei 071001; 2.Landscape Department, Hebei professional College of Political Science and Law, Shijiazhuang 050061; 3College of Forestry, Agricultural University of Hebei, B aoding Heibei 071001; 4College of Horticulture, Agricultural University of Hebei, Baoding Hebei 071001 ) |
| Abstract: | Taking stem as the primary explants, a tissue culture system was established by using medium added with hormone at different concentration for two provenances of Siberian elm obtained from Cangzhou, Hebei; Chifeng, Neimeng. The results showed that the best differentiation medium for stem from Cangzhou is MS +6-BA 0.10㎎/L+IBA0.005㎎/L and for stem from Chifeng is MS+6-BA 0.10㎎/L+IBA0.025㎎/L; the best rooting medium for stem from Cangzhou is MS +IBA0.01㎎/L and for stem from Chifeng is MS +IBA0.05㎎/L. MS medium supplemented with 0.005mg/L TDZ and 0.005㎎/L IAA was the optimal medium condition of leaf regeneration of these two Siberian elms. |
| Keywords: | Siberian elm tissue culture provenance leaf regeneration |
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