| 应用气相色谱仪分析油菜脂肪酸含量 |
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| 引用本文： | 高建芹,浦惠明,戚存扣,张洁夫,陈新军,龙卫华,傅寿仲.应用气相色谱仪分析油菜脂肪酸含量[J].江苏农业学报,2008,24(5). |
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| 作者姓名： | 高建芹 浦惠明 戚存扣 张洁夫 陈新军 龙卫华 傅寿仲 |
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| 作者单位： | 江苏省农业科学院经济作物研究所,国家油菜改良江苏分中心,江苏,南京,210014 |
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| 基金项目： | 国家引进国际先进农业科学技术计划(948计划)，国家支撑计划项目 |
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| 摘 要： | 运用Agilent 6890N GC对具有不同脂肪酸组分的油菜样品,采用半粒、单粒和混合样3种测试方法进行分析,建立适合于油菜籽各脂肪酸组分含量的定量测试程序.结果表明:在本研究设定的分析条件下,二阶程序升温条件下对油菜7种主要脂肪酸的检测效果好于恒温条件,而测试效率则恒温条件高于二阶程序升温条件下.重复性和再现性试验结果显示:在二阶程序升温和恒温条件下半粒种子、单粒种子和混合样3种方法测试的脂肪酸含量大于5%的组分和小于5%的组分重复间绝对误差、相对误差均小于国家标准,表明这3种方法重复性、再现性好,能满足不同品质材料、不同检测要求的全脂肪酸组分的定量分析需要.
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| 关 键 词： | 气相色谱仪 分析方法 油菜脂肪酸含量 |
Analysis of Fatty Acid Content in Rapeseed by Gas Chromatography |
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| GAO Jian-qin,PU Hui-ming,QI Cun-kou,ZHANG Jie-fu,CHEN Xin-jun,LONG Wei-hua,FU Shou-zhong.Analysis of Fatty Acid Content in Rapeseed by Gas Chromatography[J].Jiangsu Journal of Agricultural Sciences,2008,24(5). |
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| Authors: | GAO Jian-qin PU Hui-ming QI Cun-kou ZHANG Jie-fu CHEN Xin-jun LONG Wei-hua FU Shou-zhong |
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| Institution: | GAO Jian-qin,PU Hui-ming,QI Cun-kou,ZHANG Jie-fu,CHEN Xin-jun,LONG Wei-hua,FU Shou-zhong(Institute of Industrial Crops,Jiangsu Branch of National Rapeseed Improvement Center,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China) |
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| Abstract: | To establish an analytical procedure suitable for measuring fatty acid composition of rapeseeds,half-,single-and mixed-seed of rapeseeds with different fatty acids composition were measured using Agilent 6890 N GC.The test was led to a better result at the two-order programmed temperature,which has a good diagram with sharp and narrow peaks,excellent column effect and resolution,than that at the constant temperature.However,the constant temperature had a better testing efficiency compared to the programmed ... |
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| Keywords: | gas chromatography analytical procedure fatty acid content in rapeseed |
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