丽格海棠再生体系的建立

目的]研究丽格海棠组织培养再生植株的最佳培养基配方条件,为其快速繁殖提供技术参考。方法]以丽格海棠幼嫩叶片为外植体,对其进行诱导、增殖和生根培养,确定其再生体系。结果]以1 cm2左右带叶脉的幼嫩叶片为外植体,诱导愈伤和丛生芽的培养基为MS＋6-BA 0.50 mg/L＋NAA 0.50 mg/L＋4%芦荟原汁,诱导率分别为94.44%和66.67%;生根培养基为1/2MS＋NAA 0.10 mg/L,生根率为100%。结论]该研究建立了丽格海棠离体再生体系。

Establishment of Regeneration System of Rieger begonia

Objective] The aim was to study the best medium formula for the tissue culture of regenerated plants of Rieger begonia so as to provide technical references for its rapid propagation.Method] With the young leaves of Rieger Begonia as explants,which were induced,proliferated and rooting cultured in order to determine its regeneration system.Result] With the Rieger begonia leaf of about 1 cm2 with main vein as explants,the suitable medium for callus and adventitious shoot induction was MS+6-BA 0.50 mg/L+NA...