| 不同甘油浓度下稀释液中添加棉子糖对绒山羊冷冻精液品质的影响 |
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| 引用本文： | 张建新,白元生,刘晓妮,李志军,焦光月,赵宇琼,李鹏. 不同甘油浓度下稀释液中添加棉子糖对绒山羊冷冻精液品质的影响[J]. 中国畜牧杂志, 2012, 48(5): 22-26 |
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| 作者姓名： | 张建新 白元生 刘晓妮 李志军 焦光月 赵宇琼 李鹏 |
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| 作者单位： | 1. 山西农业大学动物科技学院,山西太谷,030801
2. 山西省生态畜牧产业管理站,山西太原,030001 |
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| 摘 要： | 在甘油浓度为3%和6%的冷冻精液稀释液中添加不同浓度的棉子糖,旨在优化绒山羊冷冻稀释液配方。结果表明:甘油浓度为3%时,山羊精液稀释液的抗冻保护效果较好,在此基础上添加10 mmol/L棉子糖,精子活率、顶体完整率、生存指数、质膜完整率均高于其他实验组(P<0.05),并且畸形率显著降低(P<0.05);在脂质过氧化反应和抗氧化酶活性方面,该实验组CAT活性高于其他实验组(P<0.05),而MDA浓度低于其他实验组(P<0.05)。
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| 关 键 词： | 冷冻精液 稀释液 甘油 棉子糖 绒山羊 |
The Effect of Supplementation of Raffinose on the Quality of Cashmere Goat Frozen Sperm Dilution Containing Glycerol of Different Doncentration |
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| ZHANG Jian-xin , BAI Yuan-sheng , LIU Xiao-ni , LI Zhi-jun , JIAO Guang-yue , ZHAO Yu-qiang , LI Peng. The Effect of Supplementation of Raffinose on the Quality of Cashmere Goat Frozen Sperm Dilution Containing Glycerol of Different Doncentration[J]. Chinese Journal of Animal Science, 2012, 48(5): 22-26 |
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| Authors: | ZHANG Jian-xin BAI Yuan-sheng LIU Xiao-ni LI Zhi-jun JIAO Guang-yue ZHAO Yu-qiang LI Peng |
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| Affiliation: | 1.College of Animal Science and Technology,Shanxi Agricultural University,Shanxi Taigu 030801,China; 2.Management Station of Ecological Stockbreeding of Shanxi Province,Shanxi Taiyuan 030001,China) |
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| Abstract: | Raffinose was added to the frozen semen dilution containing glycerol of different concentration to determine a better formula for the preservation of cashmere goat spermatozoa.The results demonstrated that 3% glycerol provided a better protective efficacy.When supplemented 10mmol/L raffinose to it,sperm viability,acrosome integrity rate,survival index,rate of plasma membrane integrity were significantly higher than other combinations(P<0.05),and abnormality rate was markedly lowered(P<0.05).As far as the lipid peroxidation and antioxidant enzyme activities were concerned,CAT activity was significantly higher and MDA content was markedly lower than other treatments(P<0.05). |
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| Keywords: | cashmere goat frozen semen diluent glycerol raffinose |
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