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| 绵羊催乳素受体基因外显子10多态性及其与产羔数相关分析 | |
| 引用本文： | 张向楠,孙伟,储明星,张有法,陈玲,吴文忠,周洪,徐厚生.绵羊催乳素受体基因外显子10多态性及其与产羔数相关分析[J].中国畜牧杂志,2012,48(17):1-6. |
| 作者姓名： | 张向楠 孙伟 储明星 张有法 陈玲 吴文忠 周洪 徐厚生 |
| 作者单位： | 1. 扬州大学动物科学与技术学院,江苏扬州,225009 2. 中国农业科学院北京畜牧兽医研究所,农业部畜禽遗传资源与种质创新重点实验室北京,100193 3. 苏州市畜牧兽医站,江苏苏州,215200 4. 徐州市睢宁县林牧渔业局,江苏睢宁,221200 |
| 基金项目： | 现代肉羊产业技术体系建设，中国博士后科学基金特别资助项目，科技部家养动物种质资源平台，江苏省农业科技支撑计划项目，江苏高校优势学科建设工程资助项目，国家高技术研究发展计划(863计划)，江苏省苏北科技发展计划 |
| 摘 要： | 采用PCR-SSCP技术检测催乳素受体(PRLR)基因外显子10在湖羊、小尾寒羊、洼地羊和阿勒泰羊中的单核苷酸多态性,研究该基因与湖羊高繁殖力的关联性。结果表明:4个绵羊品种在P2引物扩增区域均符合Hardy-Wenberg平衡,但P3引物只有阿勒泰羊符合Hardy-Wenberg平衡。P2扩增片段的不同基因型分布频率在4个品种间差异不显著(P0.05);而除阿勒泰羊P3扩增片段的基因型分布与其余3个品种间存在极显著差异(P0.01)外,其余各品种间基因型分布差异不显著(P0.05)。4个绵羊品种间产羔数存在极显著差异(P0.01),但不同基因型间产羔数差异不显著(P0.05);湖羊不同引物不同基因型产羔数的差异亦均不显著(P0.05)。本研究表明,PRLR基因不能作为湖羊高繁殖力的候选基因。 |
| 关 键 词： | 催乳素受体基因 多态性 产羔数 绵羊 |
Analysis on Genetic Polymorphism of Exon 10 of Prolactin Receptor Gene and Its Relationship with Litter Size of Sheep |
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| ZHANG Xian-gnan , SUN Wei , CHU Ming-xing , ZHANG You-fa , CEHN Ling , WU Wen-zhong , ZHOU Hong , XU Hou-sheng.Analysis on Genetic Polymorphism of Exon 10 of Prolactin Receptor Gene and Its Relationship with Litter Size of Sheep[J].Chinese Journal of Animal Science,2012,48(17):1-6. | |
| Authors: | ZHANG Xian-gnan SUN Wei CHU Ming-xing ZHANG You-fa CEHN Ling WU Wen-zhong ZHOU Hong XU Hou-sheng |
| Institution: | 1.College of Animal Science & Technology,Yangzhou University,Jiangsu Yangzhou 225009,China; 2.Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China; 3.Animal Science & Veterinary Medicine Bureau of Suzhou city,Jiangsu Suzhou 215128,China; 4.Forestation,Herding,Fishing Bureau of Suining County of Xuzhou City,Jiangsu Suining 221200,China) |
| Abstract: | Prolactin receptor(PRLR)gene was studied as a candidate gene for the prolificacy of Hu sheep.Primers were designed to detect single nucleotide polymorphisms of exon 10 of PRLR gene in Hu sheep,Small Tail Han sheep,Wadi sheep and Altay sheep by PCR-SSCP.The products amplified by P2 of the four sheep and P3 of Altay sheep were accorded with Hardy-Wenberg equilibrium,and others were in Hardy-Wenberg disequilibrium.In the fragment amplified by primer P2 there was no significant difference for the distribution frequency among different genotypes of the four sheep breeds(P> 0.05).In the fragment amplified by primer P3 there was extremely significant difference for the distribution frequency between Altay sheep and the other three breeds(P<0.01),and there were no significant differences among other three breeds(P> 0.05).There were extremely significant difference for the litter size among four breeds(P<0.01),but no significant difference was observed among different genotypes amplified by primer P2 and primer P3(P>0.05).There was no significant difference among the different genotypes for the litter sizes of Hu sheep(P>0.05).The research showed that PRLR gene couldn’t be selected as a candidate gene for the prolificacy of Hu sheep. |
| Keywords: | PRLR gene polymorphism litter size sheep |
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