鸵鸟卵黄低密度脂蛋白对猪精子冻后品质的影响

在家畜精液冷冻中,卵黄被广泛应用,且其中的低密度脂蛋白(LDL)对精子起主要保护作用。本研究利用含6%、7%、8%和9%鸵鸟卵黄LDL配制的稀释液制作猪细管冷冻精液,分析鸵鸟卵黄LDL对冷冻-解冻后猪精子质量参数的影响。结果表明:在含不同浓度鸵鸟卵黄LDL的稀释液中,8%LDL的稀释液冷冻效果最好,冻后精子活率平均可达52.13%,显著高于其他组(P<0.05);精子顶体完整率平均为58.33%,质膜完整率为72.38%,与其他处理组相比差异显著(P<0.05)。但与鸡蛋卵黄LDL和鸽子蛋卵黄LDL处理组相比,鸵鸟卵黄LDL处理组冷冻-解冻后猪精子质量参数相对较低。本研究表明,虽然鸵鸟卵黄LDL在冷冻过程中对猪精子具有一定的保护作用,但相对于鸽子蛋和鸡蛋卵黄LDL效果并不理想。

Effects of Ostrich Egg Yolk LDL on Boar Spermatozoa Quality Following Freezing-Thawing

Egg yolk has been widely used in boar frozen semen dilution and the protective action of yolk is largely attributed to the low-density lipoprotein(LDL).In this study,LDL of ostrich egg yolk was added at concentrations of 6-9% to the extenders used to freeze boar semen and its effects on the quality of frozen-thawed sperm were assessed.The results indicated that supplementation of LDL at 8% LDL resulted in significantly higher spermatozoa motility(52.13%) than that of other groups(P < 0.05),and there was higher acrosome integrity(58.33%) and membrane integrity(72.38%)(P < 0.05) compared with other treatment groups.But the quality parameters of boar sperm after freezing-thawing following the LDL of ostrich egg yolk is lower compared with the extender containing LDL of egg yolk and pigeon yolk.According to all measured parameters,the extender containing 8% LDL of ostrich egg yolk showed beneficial cryoprotective effects on frozen-thawed boar spermatozoa.But its effect is not best compared with 9% egg yolk LDL and 8% pigeon egg yolk LDL.