| 牛乳腺干细胞核移植 |
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| 引用本文： | 李吉霞,张涌,王勇胜,闫晶.牛乳腺干细胞核移植[J].上海交通大学学报(农业科学版),2010,28(2). |
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| 作者姓名： | 李吉霞 张涌 王勇胜 闫晶 |
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| 作者单位： | 西北农林科技大学动物医学学院生物工程研究所,杨凌,712100 |
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| 基金项目： | 国家"863";计划项目 |
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| 摘 要： | 近些年来,体细胞核移植虽然取得了很大的进步,但核移植效率仍然很低,而供核细胞的选择对于核移植效率起着关键的作用.本研究针对牛乳腺干细胞(mammary stem cells,MSCs)和牛乳腺上皮细胞(mammary epithelial cells,MECs)作为核移植的供核细胞,比较了重构胚发育率和核移植胚胎干细胞分离率的差异,发现来源于MSCs的重构胚的卵裂率为69%(331/479),囊胚发育率为27%(130/479);来源于MECs的卵裂率为71%,囊胚发育率为17%(84/495).囊胚发育率差异显著(p＜0.05).试验从176个核移植重构囊胚分离培养胚胎干细胞(embryonic stem cells,ES cells),发现来源于MSCs的重构胚的NTES贴壁率为44%(39/88),来源于MECs的NTES贴壁率为28%(25/88),NTEs的贴壁率差异显著(p＜0.05).以上数据表明,来源于MSCs的重构胚具有较高的发育潜能.
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| 关 键 词： | 乳腺干细胞 乳腺上皮细胞 胚胎干细胞 核移植 |
Nuclear Transfer from Bovine Mammary Stem Cells |
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| LI Ji-xi,ZHONG Yong,WANG Yong-sheng,YAN Jing.Nuclear Transfer from Bovine Mammary Stem Cells[J].Journal of Shanghai Jiaotong University (Agricultural Science),2010,28(2). |
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| Authors: | LI Ji-xi ZHONG Yong WANG Yong-sheng YAN Jing |
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| Institution: | LI Ji-xia,ZHONG Yong,WANG Yong-sheng,YAN Jing (Institute of Biotechnology,College of Veterinary Medicine,Northwest A&F University,Yangling 712100,China) |
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| Abstract: | Although much progress has been achieved in nuclear transfer (NT) recent years; the efficiency of the clone is still low. Donor cell selection is critical for the success of cloning animal, and it is an important factor affecting the cloning efficiency. The objective of the present work was to compare the efficiency of NT in bovine when mammary stem cells (MSCs) and mammary epithelial cells (MECs) were used as the donor cells, including embryo reconstruction, culture in vitro to the blastocyst stage and pro... |
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| Keywords: | mammary stem cells mammary epithelial cells embryonic stem cells nuclear transfer |
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