成都麻羊种群微卫星标记遗传多样性评估

作为优良的地方山羊品种,成都麻羊对我国山羊品种的改良和新品种培育起了重要作用。畜禽遗传资源本质上是基因资源,保护畜禽遗传资源就是保护基因的多样性。为了了解成都麻羊的遗传情况,通过微卫星标记技术,选用8对中高度多态微卫星标记进行成都麻羊圈养种群的遗传多样性评估,共检测到60个等位基因,平均等位基因和平均有效等位基因数分别为7.5、2.36,平均观察杂合度、平均期望杂合度和平均多态信息含量分别为0.577、0.634和0.580。研究结果表明成都麻羊种群遗传多样性较高,具有较高的保种潜力。

Genetic Diversity Assessment of Chengdu Gray Goat Based on Microsatellite Markers

Chengdu gray goat is an excellent local goat breed,which plays an important role in the improvement of goat breeds and the cultivation of new breeds in China.However,due to the lack of scientific breeding methods,some captive populations of Chengdu gray goat lead to the confusion of pedigree and inbreeding,which,to some extent,will lead to the degradation of the captive population.In the long run,the genetic diversity of Chengdu gray goat will gradually lose and some unique important genetic resources will gradually disappear.In this study,the genetic diversity assessment of Chengdu gray goat was conducted using the microsatellite markers.The results were as follows:(1)Eight pairs of microsatellite markers with medium and high polymorphism were successfully developed by cross-species method;(2)A total of 60 alleles were detected,with an average allele of 7.5,an average effective allele number of 2.36,an average observed heterozygosity of 0.577,an average expected heterozygosity of 0.634,and an average polymorphism information content of 0.580.The results showed that the genetic diversity of Chengdu gray goat population was high and it has high conservation potential.