| 抗菌肽Defensin基因pcr3.1真核表达载体的构建 |
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| 引用本文： | 张守纯,刘潇然,郭宇航.抗菌肽Defensin基因pcr3.1真核表达载体的构建[J].湖北农业科学,2009,48(1). |
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| 作者姓名： | 张守纯 刘潇然 郭宇航 |
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| 作者单位： | 沈阳农业大学畜牧兽医学院,沈阳,110161 |
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| 摘 要： | 通过克隆果蝇的defensin基因,构建了defeBSin基因的哺乳动物细胞真核表达栽体.把黑腹果蝇基因组DNA通过PCR获得defensin,克隆载体pMD18-T/defeBSin,并将其转化到大肠杆菌里.最后进行抗菌肽基因重组真核表达载体pcr3.1/defensin的构建和鉴定.结果表明,以果蝇总DNA为模板扩增出280bp左右的特异性条带,测序结果与GeneBank测序结果相比,除一个碱基外,其余的完全一致,译成的氨基酸序列相同.对重组质粒pcr3.1/defensin进行双酶切鉴定并测序.结果也完全一致.所以,重组质粒per3.1/defensin由真核载体pcr3.1和defensin DNA片段组成.且插入的defensin DNA片段与已知序列完全相同.
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| 关 键 词： | 抗菌肽 defensin基因 真核表达载体构建 |
Construction of Eukaryotic Expression Vector of Antimicrobial Peptide Gene Defensin |
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| ZHANG Shou-chun,LIU Xiao-ran,GUO Yu-hang.Construction of Eukaryotic Expression Vector of Antimicrobial Peptide Gene Defensin[J].Hubei Agricultural Sciences,2009,48(1). |
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| Authors: | ZHANG Shou-chun LIU Xiao-ran GUO Yu-hang |
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| Institution: | Animal Science Department;Shenyang Agriculture University;Shenyang 110161;China |
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| Abstract: | The eukaryotic expression vector of defensin was constructed according to the defensin gene of drosophila.De-fensin gene was obtained from drosophila genome DNA by PCR.Vector pMD18-T / defensin was cloned and transformed into Escherichia coli,and the recombinant pcr3.1 / defensin was constructed and identified.The result showed that 280 bp specif-ic fragment was amplified and identitied.Sequencing result showed that the reading frame was not changed except one base compared with the GeneBank,and amino acid ... |
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| Keywords: | PCR |
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