Gynogenesis and sex determination in large-scale loach Paramisgurnus dabryanus (Sauvage) |
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Authors: | Cuihong You Xiaomu Yu Deqing Tan Jingou Tong |
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Affiliation: | (1) State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan, 430072, China;(2) Graduate School of the Chinese Academy of Sciences, Beijing, 100039, China |
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Abstract: | Gynogenesis was induced using heterologous sperms in large-scale loach, Paramisgurnus dabryanus (Sauvage), in which a ZW/ZZ sex determination was previously proposed. Three microsatellite loci were used to monitor exclusive maternal inheritance of gynogenetic progenies. The results showed that high percentages of meiogynogens were produced at 4 min post-fertilization and mitogynogens were produced at 18 min post-fertilization by heat shocks, while meiotic gynogenesis was induced by cold shocks within a wide period and high heterozygosity was even observed in gynogens produced at 24 min post-fertilization. The sex ratios of the F1 progenies in three gynogenetic families were significantly deviated from 1:1 expectation with a female bias in two families and a male bias in one family (P < 0.05), and the other four gynogenetic families showed approximate 1:1 sex ratios. Moreover, the self-mating between gynogenetic F1 progenies and mating between gynogenetic F1 progenies and normal individuals produced all-female progenies or identical proportions of females and males. The data of sex ratios generally confirmed that the sex determination in large-scale loach was determined by the putative ZW/ZZ system, and the possible reasons causing the biased sex ratios are discussed. |
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Keywords: | Large-scale loach (Paramisgurnus dabryanus) Temperature shock Gynogenesis Sex ratio Sex determination |
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