| 永川豆豉中高产蛋白酶菌株的筛选鉴定及液体培养条件的研究 |
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| 引用本文： | 苗兰兰,曹龙奎,吴泽柱. 永川豆豉中高产蛋白酶菌株的筛选鉴定及液体培养条件的研究[J]. 农产品加工．学刊, 2009, 0(5) |
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| 作者姓名： | 苗兰兰 曹龙奎 吴泽柱 |
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| 作者单位： | 黑龙江八一农垦大学,食品学院黑龙江省农产品加工工程技术研究中心,黑龙江,大庆,163319 |
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| 摘 要： | 从永川豆豉中筛选、鉴定高产蛋白酶菌株,并对菌株的液体培养条件进行了研究。采用酪蛋白平板法,从永川豆豉中初步筛选出68株产蛋白酶菌株,通过比较发酵后蛋白酶活力,筛选出1株蛋白酶活达1468.7U/mL的高产菌株A-4。依据菌种形态和其生理生化特征,将其鉴定为枯草芽孢杆菌(Bacillus subtilis)。研究液体培养条件对菌株生长的影响,确定菌种液体培养的最佳条件为:温度34℃,pH值为8,接种量4%,菌龄12h。
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| 关 键 词： | 蛋白酶 菌种筛选 枯草芽孢杆菌 液体培养 永川豆豉 |
Yongchuan Fermented in High-yield Strains of Protease Screening and Identification of Liquid Culture Studies |
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| Miao Lanlan,Cao Longkui,Wu Zezhu. Yongchuan Fermented in High-yield Strains of Protease Screening and Identification of Liquid Culture Studies[J]. Nongchanpin Jlagong.Xuekan, 2009, 0(5) |
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| Authors: | Miao Lanlan Cao Longkui Wu Zezhu |
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| Affiliation: | Heilongjiang Agri-food Processing Development Centre;College of Food;Heilongjiang August-first Land Reclamation University;Daqing;Heilongjiang 163319;China |
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| Abstract: | Yongchuan in lobster sauce from the screening,identification of high-yield strains of protease,and strain liquid culture conditions were studied.Using casein plate,Yongchuan from fermented in a preliminary screening of 68 strains of protease production by fermentation after the protease activity compared with a selected protease 1468.7 U/mL live up to high-yield strains of A-4.Strain through morphological,physiological and biochemical characteristics identified as Bacillus subtilis(Bacillus subtilis).Throug... |
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| Keywords: | protease screening of bacteria Bacillus subtilis liquid culture Yongchuan lobster sauce |
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