首页 | 本学科首页   官方微博 | 高级检索  
     

永川豆豉中高产蛋白酶菌株的筛选鉴定及液体培养条件的研究
引用本文:苗兰兰,曹龙奎,吴泽柱. 永川豆豉中高产蛋白酶菌株的筛选鉴定及液体培养条件的研究[J]. 农产品加工.学刊, 2009, 0(5)
作者姓名:苗兰兰  曹龙奎  吴泽柱
作者单位:黑龙江八一农垦大学,食品学院黑龙江省农产品加工工程技术研究中心,黑龙江,大庆,163319
摘    要:从永川豆豉中筛选、鉴定高产蛋白酶菌株,并对菌株的液体培养条件进行了研究。采用酪蛋白平板法,从永川豆豉中初步筛选出68株产蛋白酶菌株,通过比较发酵后蛋白酶活力,筛选出1株蛋白酶活达1468.7U/mL的高产菌株A-4。依据菌种形态和其生理生化特征,将其鉴定为枯草芽孢杆菌(Bacillus subtilis)。研究液体培养条件对菌株生长的影响,确定菌种液体培养的最佳条件为:温度34℃,pH值为8,接种量4%,菌龄12h。

关 键 词:蛋白酶  菌种筛选  枯草芽孢杆菌  液体培养  永川豆豉

Yongchuan Fermented in High-yield Strains of Protease Screening and Identification of Liquid Culture Studies
Miao Lanlan,Cao Longkui,Wu Zezhu. Yongchuan Fermented in High-yield Strains of Protease Screening and Identification of Liquid Culture Studies[J]. Nongchanpin Jlagong.Xuekan, 2009, 0(5)
Authors:Miao Lanlan  Cao Longkui  Wu Zezhu
Affiliation:Heilongjiang Agri-food Processing Development Centre;College of Food;Heilongjiang August-first Land Reclamation University;Daqing;Heilongjiang 163319;China
Abstract:Yongchuan in lobster sauce from the screening,identification of high-yield strains of protease,and strain liquid culture conditions were studied.Using casein plate,Yongchuan from fermented in a preliminary screening of 68 strains of protease production by fermentation after the protease activity compared with a selected protease 1468.7 U/mL live up to high-yield strains of A-4.Strain through morphological,physiological and biochemical characteristics identified as Bacillus subtilis(Bacillus subtilis).Throug...
Keywords:protease  screening of bacteria  Bacillus subtilis  liquid culture  Yongchuan lobster sauce  
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号