镉胁迫对葡萄砧木5BB和99R组培苗生长的影响

利用组织培养技术,研究了不同质量浓度镉(Cd)(0、1、3、5mg/L)对2个葡萄砧木品种(5BB和99R)的生长特性及Cd积累规律的影响。结果表明,随着Cd质量浓度的增加,2个葡萄砧木品种的生物量、株高、根系长度和表面积均呈现下降的趋势,且99R的下降幅度明显高于5BB。随着Cd质量浓度的增加,2个葡萄砧木品种Cd含量明显增加,在试验设置的Cd质量浓度条件下,5BB地上部和根系Cd含量均低于99R。2个葡萄砧木品种对Cd胁迫的反应虽然总体趋势一致,但5BB的生物量、株高和根系形态指标的下降幅度明显低于99R,表明5BB比99R具有较强的耐Cd特性。

Effect of Cadmium Stress on Growth Characteristics of Two Tissue Cultured Grapevine Rootstocks 5BB and 99R

The experiment was carried out to study the growth characteristics and regular of cadmium(Cd) accumulation in roots and shoots by using tissue-cultured grapevine rootstocks(5BB and 99R) under Cd spiked at 0,1,3,5 mg/L.The results showed that biomass,plant height,root length and surface area of two grapevine rootstocks decreased with the increase of Cd concentration in culture medium,and their effect on 99R was more significant than on 5BB.The Cd concent in grapevine seedling increased significantly with the increase of Cd concentration in culture medium,and its concent in roots and shoots of 5BB was lower than in 99R.The decreased ranges of biomass,plant height and root morphological parameters of 5BB under Cd treatments were much smaller than those of 99R,indicating that 5BB had a higher ability to tolerate Cd stress than 99R.