家蚕抗菌肽enbocin基因的一种异常选择性剪接方式

抗菌肽是昆虫抵御外界微生物侵染的主要物质。研究了家蚕经大肠杆菌(JM109)、农杆菌(LBA4404)和家蚕核型多角体病毒(BmNPV)3种外源微生物诱导后,抗菌肽enbocin基因在蚕体血液和脂肪体中的表达。3种外源微生物诱导前、后,抗菌肽enbocin基因在蚕体血液中的表达均较弱。在蚕体脂肪体中,抗菌肽enbocin基因的表达在BmNPV诱导前、后差异不大;而JM109、LBA4404菌株诱导后则均出现特异性表达,且特异表达形式的表达量与正常表达具有补偿作用。克隆测序正常转录和诱导后特异转录的条带(GenBank:FJ373019),发现特异转录形式是由于正常表达形式的外显子/内含子的剪接位点发生变化,使2个外显子的序列均包括了中间内含子的部分序列所致,序列的改变也使得特异转录的抗菌肽enbocin基因的翻译发生提前终止,缺失了基因C末端cecropin结构序列。

An Unusual Alternative Splicing of enbocin in Silkworm,Bombyx mori

Antimicrobial peptides are the most important substance for insect in defending themselves from infection of microorganism.In this study,three kinds of microorganisms,E.coli JM109,Agrobacterium LBA4404 and Bombyx mori nucleopolyhedrovirus(BmNPV),were used to infect silkworms and study the inductive expression of enbocin gene in silkworm hemocyte and fat body.The results showed that,in hemocyte,there was low level expression in all the treatments;and in fat body,there was very little difference in its expres...