| 芥蓝BoPGIP2全长基因的分子克隆及其序列分析 |
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| 引用本文： | 张弢.芥蓝BoPGIP2全长基因的分子克隆及其序列分析[J].农业科学与技术,2009,10(4):91-95,104. |
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| 作者姓名： | 张弢 |
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| 作者单位： | 青岛农业大学生命科学学院,山东青岛,266109 |
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| 基金项目： | School High-level Talent Starting Fund of Qingdao Agriculture University: Studies on Clone and Evolution of PGIP Gene from Brassica crops (630745).青岛农业大学校高层次人才启动基金:芸苔属作物PGIP基因的克隆及其进化研究 |
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| 摘 要： | 1材料与方法
1．1材料与试剂试材为“中花”芥蓝，种子播于装有蛭石和珍珠岩各半的苗钵中，并在玻璃温室中生长。幼苗期取幼嫩叶片，用75％的酒精棉擦洗干净，做好标记，立即投入液氮中固定，-75℃保存备用。
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| 关 键 词： | 序列分析 分子克隆 全长基因 芥蓝 玻璃温室 幼嫩叶片 珍珠岩 幼苗期 |
Molecular Cloning and Sequence Analysis of BoPGIP2 Gene from Brassica oleracea L. var. alboglabra |
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| ZHANG.Molecular Cloning and Sequence Analysis of BoPGIP2 Gene from Brassica oleracea L. var. alboglabra[J].Agricultural Science ＆ Technology,2009,10(4):91-95,104. |
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| Authors: | ZHANG |
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| Institution: | ZHANG Tao ( Life Science College, Qingdao Agriculture University, Qingdao 266109) |
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| Abstract: | PGIP gene was obtained from Brassica oleracea L. var. alboglabra, named BoPGIP2. The full length of BoPGIP2 gene is 1 102 bp and the exon is 993 bp which encodes a protein of 330 amino acids with a predicted molecular mass of 37.1 kDa, interrupted by one intron of 95 bp in, length. Sequence analysis revealed that it has five potential N-giycosylation sites, two protein kinase C phosphrylation sites, five casin kinase Ⅱ phosphrylation sites and four N-myristoylation sites. The amino acids sequences alignment confirmed that ^145 LRR stucture was highly conserved in all aligned PGIP sequences. |
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| Keywords: | Brassica oleracea L var alboglabra BoPGIP2 Molecular cloning Sequence analysis |
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