小鼠睾丸支持细胞分离培养及生物学特性鉴定

为了揭示睾丸支持细胞在精子发生及睾丸免疫豁免中的生物学作用,采用胰蛋白酶、胶原酶分步消化法和1/3浓度的Hank′s低渗液、50mmol/L Tris-HC(l pH7.1)液分别低渗处理及油红O脂质染色法对16～22d的幼鼠睾丸进行分离、纯化和生物学特性鉴定。结果表明:分离、纯化后的睾丸支持细胞损伤少、活率高、睾丸支持细胞占培养细胞总数的90%以上;根据形态学观察及油红O脂质染色法鉴定,睾丸支持细胞的生长状态良好,细胞突起很多,核仁清晰,胞质中可见大小不等的红色空泡状脂质小滴。

Isolation, Purification and Identification of Sertoli Cells in Prepubertal Mouse

In order to reveal sertoli cells play a vital biological role in spermatogenesis and immune privilege,mouses sertoli cells were isolated,purified and identified by steps digestion with trypsin and collagenase,low osmotic treatment with the Hank's hyposmolality liquid(1/3 concentration) and 50 mmol/L Tris-HCl(pH 7.1)and oil red O staining.The results showed that isolated and purified sertoli cell had small damnification and high livability,cell purity could reached 90%;Sertoli cells had good cell viability,nucleohs were clear,grains and vacuoles could be observed in the cytoplasm with morphological observe and Oil Red O staining.