GA3和IAA对慈竹黄酮含量动态积累的调控效应

[目的]研究GA3和IAA对慈竹茎和叶黄酮含量动态积累的调控作用,为调控慈竹茎叶中黄酮类化合物合成提供理论依据。[方法]以慈竹为材料,通过向慈竹喷施不同浓度与配比的GA3和IAA溶液,采用NaNO2-Al(NO3)3比色法测定慈竹茎和叶的黄酮含量。[结果]200mg/LGA3+50mg/LIAA处理和对照的慈竹茎黄酮含量的动态变化呈现高-低-高的"V"字形变化趋势,50mg/LGA3+200mg/LIAA处理茎黄酮含量的积累呈增长的趋势。GA3和IAA处理没有改变慈竹叶片黄酮含量的动态积累趋势,与对照基本一致,都是先上升后急剧下降至最低谷,然后迅速回升。GA3和IAA处理对慈竹茎和叶片黄酮含量动态积累的调节作用与浓度和配比有关。不同浓度和配比的GA3和IAA处理对慈竹茎黄酮的合成都有抑制作用;高浓度GA3和低浓度IAA配比对慈竹叶片黄酮的合成有抑制作用。对茎和叶黄酮合成的抑制作用都在处理的前期较为明显。低浓度GA3和高浓度IAA配比对慈竹叶黄酮的合成具有促进作用。[结论]该研究为进一步开发慈竹提供了理论依据。

Effects of GA3 and IAA on the Dynamic Accumulation of Flavonoid Content in Neosinocalamus affinins

[Objective] The research aimed to study the regulation of GA3 and IAA on the dynamic accumulation of flavonoid contents from leaves and stem in Neosinocalamus affinins and to provide theories for regulating the flavonoid biosynthesis in bamboo. [Method] Taking Neosinocalamus affinins as material, The GA3 and IAA with the different concentrations and combinations were sprayed onto the Neosinocalamus affinins. The flavonoid contents was determined by NaNO2-Al(NO3)3 colorimetric method. [Result] The changes of dynamic accumulation of flavonoid content from stem of Neosinocalamus affinins under the treatments of CK and 200 mg/L GA3 50 mg/L IAA appeared the shape like the capital letter "V", but the treatment 50 mg/L GA3 200 mg/L IAA was increased. There was the same dynamic accumulation of flavonoid content from leaves in Neosinocalamus affinins sprayed the GA3 and IAA with the different concentrations and combinations, and all appeared the trend that the dynamic accumulation of flavonoid content from leaves firstly increased, and then decreased quickly, finally increased again. The GA3 and IAA with the different concentrations and combinations had negative effect on the flavonoid biosynthesis from stem in Neosinocalamus affinins during the early period. The high concentration GA3 combining with the low concentration IAA had negative effect on the flavonoids biosynthesis from leaves in Neosinocalamus affinins. The GA3 and IAA with the different concentrations and combinations had significant effect on the flavonoids biosynthesis from both stem and leaves in Neosinocalamus affinins during the early period. The low concentration GA3 combining with the high concentration IAA had positive effect on the flavonoi ds biosynthesis from leaves in Neosinocalamus affinins. [Conclusion] The research provided foundation for Neosinocalamus affinins exploiture.