Use of Chaetomium globosum for biocontrol of potato late blight disease |
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| Affiliation: | 1. University of New Mexico, Department of Biology, Albuquerque, NM, USA;2. Sandia National Laboratories, Albuquerque, NM, USA;3. Concordia University, Centre for Structural and Functional Genomics, Montreal, QC, Canada;4. Novozymes Inc., Davis, CA, USA;5. DOE Joint Genome Institute, 2800 Mitchell Dr, Walnut Creek, CA, USA;1. Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education and Jiangsu Province, Institute of Agricultural Engineering of Jiangsu University, 212013 Zhenjiang, China;2. School of Environment, Jiangsu University, 212013 Zhenjiang, China;3. School of Mechanics and Power Engineering, Henan Polytechnic University, Jiaozuo 454003, China;4. College of Forest Resources and Environment, Nanjing Forestry University, 210037 Nanjing, China;1. College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China;2. College of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056021, China;3. College of Life Sciences, Chongqing Normal University, Chongqing 401331, China;1. Technical University of Manabí, Faculty of Agronomic Engineering, Experimental Campus La Teodomira, Km 13, Santa Ana, Manabí, Ecuador;2. ESPOL Polytechnic University, Escuela Superior Politécnica del Litoral, ESPOL, Centro de Investigaciones Biotecnológicas del Ecuador (CIBE), Campus Gustavo Galindo Km 30.5 Vía Perimetral, P.O. Box 09-01-5863, Guayaquil, Ecuador;3. ESPOL Polytechnic University, Escuela Superior Politécnica del Litoral, ESPOL, Facultad de Ciencias de la Vida (FCV), Campus Gustavo Galindo Km 30.5 Vía Perimetral, P.O. Box 09-01-5863, Guayaquil, Ecuador;4. Biosequence, Quito, Ecuador;5. Instituto Nacional Autónomo de Investigaciones Agropecuarias (INIAP), Departamento Nacional de Recursos Fitogenéticos (DENAREF), Estación Experimental Santa Catalina, Quito, Ecuador;6. Federal University of Santa Maria, Department of Physics, Climate Research Group, Av. Roraima, 1000, Santa Maria, RS, Brazil;7. Departamento de Ciencias Biológicas, Facultad de Ciencias de la Salud, Universidad Técnica de Manabí, Portoviejo, 130105, Ecuador |
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| Abstract: | The efficacy of Chaetomium globosum as a biocontrol agent against the late blight pathogen Phytophthora infestans was evaluated in potato plants. Among eight Chaetomium isolates evaluated C. globosum isolate Cg-6 showed greater inhibition to mycelial growth of P. infestans in vitro. TLC studies showed that isolate Cg-6 produced an antibiotic called ‘Chaetomin’. Isolate Cg-6 showed greater exo- and endo-glucanase enzyme activity when compared to other isolates. PCR amplification of the ITS region and sequencing of the PCR product confirmed that isolate Cg-6 belongs to the C. globosum group. C. globosum Cg-6 was formulated as a liquid and applied as a tuber, soil and foliar treatment either individually or in combination against Phytophthora infection in potato plants. Among different treatments, combined application of C. globosum as a tuber treatment @ 1 ml/kg of tubers, as a soil application @ 1 ml/kg of Farm Yard Manure (FYM) and foliar spray @ 0.7% resulted in significantly less late blight infection (72%) compared to untreated control (100%) under field conditions. The application of C. globosum resulted in greater tuber yield by reducing late blight infection in two field trials when compared to untreated controls. The study clearly demonstrated the potential use of C. globosum as a biocontrol agent in the management of late blight disease in potato plants. |
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| Keywords: | Endoglucanase Late blight Potato |
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