In vitro compatibility of microbial agents for simultaneous application to control strawberry powdery mildew (Podosphaera aphanis) |
| |
| Institution: | 1. Geisenheim University, Institute of Phytomedicine, Von-Lade-Str. 1, DE-65366 Geisenheim, Germany;2. Swedish University of Agricultural Sciences, Department of Biosystems and Technology, Microbial Horticulture Laboratory, P.O. Box 103, SE-23053 Alnarp, Sweden;3. Geisenheim University, Institute of Pomology, Von-Lade-Str.1, DE-65366 Geisenheim, Germany;1. Viçosa Federal University, Department of Plant Pathology, Laboratory of Plant Protection, Viçosa, Minas Gerais State Zip Code 36570-000, Brazil;2. Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural, Venda Nova do Imigrante, Espirito Santo State Zip Code 29375-000, Brazil;3. Viçosa Federal University, Department Crop Science, Viçosa, Minas Gerais State Zip Code 36570-000, Brazil;1. Department of Plant Sciences, Mail Stop 2, University of California, One Shields Avenue, Davis, CA 95616-8780, USA;2. Departamento de Horticultura, Universidad Autonoma Agraria Antonio Narro, Buenavista, Saltillo, Coahuila 25315, Mexico;3. Departamento de Maquinaria Agricola, Universidad Autonoma Agraria Antonio Narro, Buenavista, Saltillo, Coahuila 25315, Mexico;1. Département de phytologie, Université Laval, Québec, QC G1V 0A6, Canada;2. Premier Tech, 1 avenue Premier, Rivière-du-Loup, QC G5R 6C1, Canada;3. Département de médecine moléculaire, Université Laval, Québec, QC G1V 0A6, Canada;4. Centre de recherche et d''innovation sur les végétaux, Université Laval, Québec, QC G1V 0A6, Canada |
| |
| Abstract: | Biological control of plant pathogens on strawberries may be improved by the simultaneous application of different biological control agents (BCAs). Therefore, the compatibility of various BCAs which had previously shown to be effective against powdery mildew (Podosphaera aphanis (Wallr.) U. Braun & S. Takam) under laboratory conditions was examined in vitro. Inhibitory effects between fungal and bacterial BCAs were demonstrated in dual culture tests on two solid nutrient media. Leaf disc assays with single and multiple strain treatments demonstrated either unaffected or significantly improved control of P. aphanis for many multiple strain treatments, even if antagonistic interactions previously occurred in dual culture tests. Highest inhibition of powdery mildew conidiation (80.7% reduction) was achieved with multiple strain treatments with Bacillus subtilis FZB24 and Metarhizium anisopliae (p < 0.001). In this combination, conidiation was 3.7 times lower than in single treatments with B. subtilis indicating synergistic interactions between these BCAs. Combinations of Trichoderma harzianum T58 and B. subtilis FZB24 showed antagonistic interactions in dual culture tests as well as in leaf disc assays. In this combination, powdery mildew conidiation on leaf discs was four times higher compared to single treatments with T. harzianum T58. |
| |
| Keywords: | Biological control BCA combinations BCA compatibility |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
