| 一种昆虫的分子生物学鉴定 |
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| 引用本文： | 龚子端,王宇,郑毅.一种昆虫的分子生物学鉴定[J].安徽农业科学,2011,39(35):21834-21835. |
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| 作者姓名： | 龚子端 王宇 郑毅 |
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| 作者单位： | 1. 攀枝花市干热河谷生物工程研究中心,四川樊枝花617000;攀枝花学院生物与化学工程学院,川攀枝花617000
2. 攀枝花市干热河谷生物工程研究中心,四川樊枝花617000;攀枝花学院医学院,四川攀枝花617000 |
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| 摘 要： | 目的]生物学鉴定攀西地区1种重要的经济昆虫。方法]采用分子生物学技术对该昆虫进行线粒体细胞色素氧化酶（COI）基因种属特异性研究。结果]利用PCR技术扩增出长度为1 100 bp的序列,经过序列分析比对,确定该昆虫属于昆虫纲,广翅目（Mega-loptera）,齿蛉科（Corydalidae）,巨齿蛉属（Acanthacorydalis）。结论]结果为人工养殖该种昆虫提供了重要的理论依据。
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| 关 键 词： | 齿蛉科 PCR扩增 线粒体细胞色素C氧化酶亚基I基因 |
Species Identification of An Insect by Molecular Characterization |
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| Institution: | GONG Zi-duan et al(Panzhihua Bio-engineering Research Center of hot-dry valley,Panzhihua,Sichuan 617000) |
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| Abstract: | Objective]The research aimed to biological indentify one species of important economy insect in Panxi area.Method]The mitochondrial cytochrome oxidase I(COI) gene sequence of this insect was studied by the molecular-biology techniques to identify its genus and species.Result]About an 1 100 bp region of mitochondrial cytochrome oxidase I gene was amplified by polymerase chain reaction(PCR).The results of the sequencing analysis and contrast confirm that the insect belongs to Acanthacorydalis,Corydalidae,Megaloptera,Insecta.Conclusion]The results present important theoretic gist for breeding this insects artificially. |
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| Keywords: | Corydalidae PCR amplification Mmitochondrial cytochrome oxidase I(COI) gene |
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