小麦秸秆发酵液中优质乳酸菌的分离·纯化与鉴定 |
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引用本文: | 阿布都克尤木·麦麦提,热娜·阿布都米吉提,乌斯满·依米提. 小麦秸秆发酵液中优质乳酸菌的分离·纯化与鉴定[J]. 安徽农业科学, 2011, 39(35): 21824-21828 |
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作者姓名: | 阿布都克尤木·麦麦提 热娜·阿布都米吉提 乌斯满·依米提 |
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作者单位: | 新疆大学生命科学与技术学院,新疆乌鲁木齐,830046 |
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基金项目: | 新疆大学自然科学基金项目 |
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摘 要: | [目的]从小麦秸秆发酵液中筛选并鉴定适合于青贮添加剂的高效乳酸菌。[方法]用MRS+CaCO3固体培养基筛选,分离出78株乳酸菌;经形态学、革兰氏染色、生理生化试验、产酸效率试验、耐酸能力试验和耐盐试验等挑选出43株产酸率高的乳酸菌。通过16S rDNA法对该乳酸菌进行鉴定。[结果]已获得43株产酸能力强的菌株1,6S rDNA鉴定结果表明,37株为副干酪乳杆菌亚种(Lacto-bacillus paracasei subsp.)1,株为鼠李糖乳杆菌(Lactobacillus rhamnosus),5株为肠球菌(Enterococcus faecium)。[结论]从小麦秸秆中筛选出43株具有较强产酸能力的乳酸菌,对研发优质青贮饲料添加剂具有一定的指导意义。
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关 键 词: | 小麦秸秆 乳酸菌 分离 鉴定 |
Isolation and Identification of High-Quality Lactic Acid Bacteria from Wheat Haulm |
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Affiliation: | Abdukiyum·Mamat et al(College of Life Sciences and Technology,Xinjiang University,Urumqi,Xinjiang 830046) |
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Abstract: | [Objective] The aim of this study was to isolate and identify lactic acid bacteria(LAB) from wheat haulm and to select efficient strains for silage fermentation.[Method] From 78 LAB strains isolated on MRS+CaCO3 solid medium,we selected 43 strains having better acid-production ability through morphological observation,Gram staining,physiological and biochemical tests,acid production test,acid tolerance test and salt tolerance test.These strains were finally identified by sequencing 16S rDNA.[Result] Of the 43 LAB strains having better acid-production ability,37 were Lactobacillus paracasei subsp.,one was Lactobacillus rhamnosusand five were Enterococcus faecium,as shown by sequences of 16S rDNA.[Conclusion] A total of 43 LAB strains having better acid-production ability were selected,which may be developed as high-quality silage additives. |
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Keywords: | Wheat haulm Lactic acid bacteria Isolation Identification |
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