| 培养基pH值对芥蓝离体再生的影响 |
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| 引用本文： | 秦耀国,杨翠芹,程明军,曹必好,陈国菊,雷建军. 培养基pH值对芥蓝离体再生的影响[J]. 中国蔬菜, 2010, 1(6): 47-49 |
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| 作者姓名： | 秦耀国 杨翠芹 程明军 曹必好 陈国菊 雷建军 |
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| 作者单位： | 1.四川农业大学园艺学院，四川雅安 625014;2.四川农业大学农学院，四川雅安 625014；;3.华南农业大学园艺学院，广东广州 510642 |
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| 基金项目： | 四川省教育厅科研项目(003227) |
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| 摘 要： | 比较不同pH值的MS培养基经高温高压灭菌后pH值的变化,并通过不同pH值处理的培养基培养芥蓝下胚轴与不定芽,探讨培养基pH值对芥蓝离体再生的影响。结果表明:不同pH值的MS培养基经高温高压灭菌后,pH值表现不同程度的下降;最适于芥蓝下胚轴分化的培养基是灭菌前pH值为6.20、灭菌后为5.65的处理,最适于生根的培养基是灭菌后pH值高于6.00的两个处理。
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| 关 键 词： | MS培养基 pH值 芥蓝 再生 |
| 收稿时间： | 2009-11-19; |
Effects of pH Value of Culture Medium on Chinese Kale (Brassica alboglabra L. H. Bailey) in Vitro Regeneration |
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| QIN Yao-guo,YANG Cui-qin,CHENG Ming-jun,CAO Bi-hao,CHEN Guo-ju,LEI Jian-jun. Effects of pH Value of Culture Medium on Chinese Kale (Brassica alboglabra L. H. Bailey) in Vitro Regeneration[J]. China Vegetables, 2010, 1(6): 47-49 |
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| Authors: | QIN Yao-guo YANG Cui-qin CHENG Ming-jun CAO Bi-hao CHEN Guo-ju LEI Jian-jun |
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| Affiliation: | 1.College of Horticulture,Sichuan Agricultural University,Ya’an 625014,Sichuan,China;2.College of Agronomy,Sichuan Agricultural University,Ya’an 625014,Sichuan,China;3.College of Horticulture,South China Agricultural University,Guangzhou 510642,Guangdong,China |
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| Abstract: | After autoclave sterilization,changes of different pH value of MS medium were compared.Hypocotyls and adventitious buds were cultured in culture medium with different pH value to discuss the effect of pH value on in vitro regeneration.The results showed that after autoclave sterilization,pH value of MS medium with different pH value declined in varying degrees.The most appropriate differentiation medium for hypocotyls of Chinese kale(Brassica alboglabra L.H.Bailey)was the treatment with 6.20 pH value before... |
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| Keywords: | MS medium pH value Chinese kale Regeneration |
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