基于体细胞组织培养的甘蓝型油菜核三系保持系绵7MB-1亲本繁殖与F1制种

1材料与方法1．1材料 核三系临保系：绵7MB-1；核两系不育系：绵7AB-4—1；恢复系：绵恢9613及品93-496（引自四川省农业科学院）。诱导培养基：B5＋谷氨酰胺2mg／L＋水解乳蛋白50mg／L＋6-BA2mg／L＋2T 1mg／L。增殖培养基：MS＋6-BA 1 mg／L＋NAA 0．1mg／L。

Reproduction of the Three-line Genic Male Sterile Line Parent Mian 7MB-1 (Brasscia Napus L.) and Seed Production of F1 Based on Somatic Tissue Culture

[Objective] The aim was to study the reproduction of the three-line genic male sterile (GMS) line parent Mian 7MB-1 (B.Napus L.) and the seed production of F1 through somatic tissue culture. [Method] Through hybridization, a new breeding material Mian 7MB-1 in three-line genic temporary maintainer line propagated by tissue culture was used to improve the sterile plant rate of rapeseed in dual-purpose recessive GMS line, such as Mian 7AB type, S45AB type, and etc. And then the variety comparative test was performed. [Result] In order to avoid some fertility restoration phenomena occurring during the process of self-reproduction, Mian 7AB was propagated in bulk with somatic tissue culture of temporary maintainer line plant stem. The propagated temporary maintainer line seedlings were applied to the breeding and seed production of net room male sterile line parent, promoting the sterile plant rate of the male sterile line parent to 91.7%-93.5%. The male sterile line parents per hectare were enough for the seed production of hybrid F1 in 7 500-15 000 hm2. [Conclusion] Compared with the original dual-purpose GMS line, the seed production ultilizing male sterile line with high sterile plant rate greatly reduced the labor, significantly improved the seed yield, ensuring the seed quality and forming a perfect breeding and seed production system.