Protein Heterogeneity in European Wheat Landraces and Obsolete Cultivars: Additional Information II |
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| Authors: | Edita Gregová Jiří Hermuth Ján Kraic Ladislav Dotlačil |
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| Institution: | (1) Department of Applied Biochemistry and Molecular Biology, Research Institute of Plant Production, Institute of Applied Genetics and Breeding, Bratislavská cesta 122, 92168 Piešt’any, Slovak Republic;(2) Research Institute of Crop Production, Gene Bank, Drnovská 507, 16106 Praha 6, Ruzyně, Czech Republic |
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| Abstract: | The endosperm storage protein of 46 European wheat (Triticum aestivum L.) landraces and obsolete cultivars have been fractionated by SDS-PAGE to determine the composition of high molecular weight
glutenin subunits (HMW-GS) composition. It has been discovered that about 46% of the wheats were heterogeneous, comprising
2–11 different glutenin profiles. Eighteen of them were observed to be homogeneous. A total of 13 HMW-GS alleles, including
3 at the Glu-A1, 8 at the Glu-B1, and 3 at the Glu-D1 loci were revealed. HMW-GS null controlled by locus Glu-A1, subunits 7 + 8 by Glu-B1, and 2 + 12 by Glu-D1 predominated. However low frequented alleles such as 17 + 18, 20, 6, and 7 were observed. Furthermore, other new alleles
encoding HMW-GS at the locus Glu-B1 have been found in one of France cultivar (Saumur d’Automne). The glutenin-based quality score ranged from 4 to 10. |
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| Keywords: | Glutenins Landraces Obsolete cultivars Storage proteins Triticum aestivum Triticum spelta Wheat |
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