水牛肺泡巨噬细胞的分离、培养及鉴定

为了深入研究水牛巨噬细胞（Macrophage,Mφ）的生物学特性,探索一种简便的水牛肺泡巨噬细胞（Alveolarmacrophage,AM）分离、培养方法,采用肺气管冷PBS灌洗法分离获得水牛肺泡细胞,并进行鉴定和功能检测。结果表明,采用肺气管冷PBS灌洗法可获得大量贴壁细胞,经光学显微镜和电子显微镜观察,其形态具备巨噬细胞的典型特征;酸性磷酸酶和非特异性酯酶阳性率分别为（89.4±3.5）%和（83.6±3.4）%;细胞表面抗原MHCⅡ阳性率为（96.6±1.3）%;鸡红细胞吞噬率为（23.6±4.2）%,墨汁吞噬率为（92.3±0.6）%。可见,肺气管冷PBS灌洗法是一种获取水牛肺泡巨噬细胞的可行方法。

Identification, isolation and cultivation of buffalo alveolar macrophage

The present experiment was conducted to establish the methodology of identification,separation,purification and cultivation of buffalo alveolar macrophage（AM） and to study the biological function of buffalo macrophage.Buffalo AM was isolated from lung-lavaging with cold PBS,the isolated cells were characterized functionally and phenotypically.The buffalo anchorage cells were obtained in large quantities which showed typical morphological characteristics of Mφ as observed by optical microscope and electron microscope.The positive rates of acid phosphatase and non-specific esterase were 89.4±3.5 and 83.6±3.4%,respectively.The positive rate of typical functional membrane proteins such as MHCⅡ was found to be 96.6±1.3%.The phagocytic rates of ink and chicken red blood cells were 92.3± 0.6 and 23.6 ± 4.2%,respectively.Therefore,the lung-lavaging with cold PBS was an efficient in vitro culture method for obtaining buffalo macrophage.