Cell wall-degrading enzymes produced in vitro by isolates of Phaeosphaeria nodorum differing in aggressiveness |
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Authors: | F. Lalaoui,P. Halama &dagger ,V. Dumortier, B. Paul |
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Affiliation: | Institut Supérieur d'Agriculture, UniversitéCatholique de Lille, 41 rue du Port, 59046 Lille, Cedex;and;Laboratoire des Sciences de la Vigne, Institut Jules Guyot, Universitéde Bourgogne, BP 138, 21004 Dijon, France |
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Abstract: | The relationships between in vitro production of cell wall-degrading enzymes and aggressiveness of three Phaeosphaeria nodorum isolates were investigated. When grown in liquid medium containing 1% cell wall from wheat leaves as the carbon source, the isolates secreted xylanase, α-arabinosidase, β-xylosidase, polygalacturonase, β-galactosidase, cellulase, β-1,3-glucanase, β-glucosidase, acetyl esterase and butyrate esterase. Time-course experiments showed different levels of enzyme production and different kinetics between isolates. A highly aggressive isolate produced more xylanase, cellulase, polygalacturonase and butyrate esterase than did the two weakly aggressive isolates. Xylanase was the most active polymer-degrading enzyme produced, suggesting a key role during pathogenesis by P. nodorum . |
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Keywords: | aggressiveness enzymes Phaeosphaeria nodorum Stagonospora nodorum |
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