| 小麦-柔软滨麦草易位系M853-4抗条锈病基因的分子标记 |
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| 引用本文： | 贺苗苗,宋晓贺,王阳,姚强,李洋,井金学. 小麦-柔软滨麦草易位系M853-4抗条锈病基因的分子标记[J]. 植物保护学报, 2010, 37(2): 118-122 |
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| 作者姓名： | 贺苗苗 宋晓贺 王阳 姚强 李洋 井金学 |
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| 作者单位： | 西北农林科技大学植物保护学院,陕西省农业分子生物学重点实验室,杨凌,712100 |
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| 基金项目： | 教育部高等学校学科创新引智计划(B07049);公益性行业(农业)科研专项(200903035-02);国家"十一五"科技支撑计划 (2006BAD08A05) |
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| 摘 要： | 为揭示小麦-柔软滨麦草易位系M853-4的抗条锈性遗传机制,以易位系M853-4和感病品种铭贤169为亲本制备F2、F3代种子,采用人工接种的方法于温室中接种小麦条锈菌生理小种Su-11,用于测定M853-4及其杂交后代的苗期抗条锈性。结果表明,M853-4对Su-11的抗病性由1对显性和1对隐性基因控制。筛选由1对显性基因控制的F3代分离家系作为SSR标记群体,从320对引物中共找到了4个位于4A染色体上的与该显性基因(暂命名为YrLm2)紧密连锁的微卫星标记Xgwm44、Xwmc650、Barc170和Xwmc718,标记到YrLm2的遗传距离分别为15.0、5.0、3.9和3.1cM,并将YrLm2定位于4A染色体的长臂上,标记结果可用于小麦分子辅助育种。
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| 关 键 词： | 小麦-柔软滨麦草易位系 抗条锈性 遗传分析 SSR 标记 |
| 收稿时间： | 2009-06-07 |
Molecular mapping of stripe rust resistance gene in wheat translocation line M853-4 derived from Leymus mollis |
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| He Miaomiao,Song Xiaohe,Wang Yan,Yao Qiang,Li Yan and Jing Jinxue. Molecular mapping of stripe rust resistance gene in wheat translocation line M853-4 derived from Leymus mollis[J]. Acta Phytophylacica Sinica, 2010, 37(2): 118-122 |
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| Authors: | He Miaomiao Song Xiaohe Wang Yan Yao Qiang Li Yan Jing Jinxue |
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| Affiliation: | College of Plant Protection, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, Shaanxi Province, China;College of Plant Protection, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, Shaanxi Province, China;College of Plant Protection, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, Shaanxi Province, China;College of Plant Protection, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, Shaanxi Province, China;College of Plant Protection, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, Shaanxi Province, China;College of Plant Protection, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, Shaanxi Province, China |
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| Abstract: | |
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| Keywords: | wheat-translocation line (Leymus mollis) stripe rust resistance genetic analysis SSR marker |
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