| 果梅离体繁殖技术研究 |
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| 引用本文： | 谭志刚,陈红,张绿萍,程秀枝.果梅离体繁殖技术研究[J].北方园艺,2010(3):118-120. |
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| 作者姓名： | 谭志刚 陈红 张绿萍 程秀枝 |
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| 作者单位： | 贵州大学,喀斯特山地果树资源研究所,贵州,贵阳,550025;贵州省果树科学研究所,贵州,贵阳,550100 |
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| 基金项目： | 贵州省科技攻关资助项目(黔科合NY字[2007]3039号);;贵州省省长基金资助项目(黔省专合字2007-18号);;贵州省果树学科科技创新人才团队建设资助项目(黔科合人才团队[2008]88007号);;贵州省特色农业产业人才培养基地建设资助项目;;贵州省果树工程技术研究中心建设资助项目(黔科合农G字[2007]4001号) |
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| 摘 要： | 以优选果梅黔荔1号单株为材料,对外植体灭菌时间、腋芽启动和增殖培养进行了研究。结果表明:以0.1%HgCl_2消毒8 min效果最好,其存活率为75%;适合腋芽启动培养的基本培养基是WPM;最有利于腋芽启动和增殖的培养基是WPM+1.0 mg/L6-BA+0.5 mg/LNAA+1.0 mg/LGA_3,有效增殖系数高达1.51。
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| 关 键 词： | 果梅 腋芽 离体繁殖 生长调节剂 |
A Preliminary Study of Prunus mume in Vitro Propagation |
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| TAN Zhi-gang,CHEN Hong,ZHANG Lv-ping,CHENG Xiu-zhi.A Preliminary Study of Prunus mume in Vitro Propagation[J].Northern Horticulture,2010(3):118-120. |
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| Authors: | TAN Zhi-gang CHEN Hong ZHANG Lv-ping CHENG Xiu-zhi |
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| Institution: | 1.Research Institute for Fruit Resources of Karst Mountain Region of Guizhou University.Guiyang;Guizhou 550025;2.Pomology Research Institute of Guizhou;Guiyang Guizhou 550100 |
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| Abstract: | Using better single plant(Qian Li NO.1) from Prunus mume as materials,explants sterilization,and proliferation culture of axillary buds were studied.The results showed that the optimal sterilization time of explant was about 8 minutes with 0.1%HgCl_2,and its survival rate was 75%.The basal medium of of axillary buds was WPM.The best medium for and proliferation of axillary buds was WPM+1.0 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L GA_3,and its effective multiplication coefficient was 1.51. |
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| Keywords: | Prunus mume axillary bud in vitro propagation plant growth regulator |
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