番鸭细小病毒与鹅细小病毒的PCR鉴别诊断 |
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引用本文: | 娄华,杨德威,贺东升. 番鸭细小病毒与鹅细小病毒的PCR鉴别诊断[J]. 中国预防兽医学报, 2000, 22(6): 459-461 |
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作者姓名: | 娄华 杨德威 贺东升 |
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作者单位: | [1]佛山科技学院动物医学系,南海 [2]华南农业大学动物医学系,广州 |
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摘 要: | 通过比较基因bank中番鸭细小病毒(MDPV)和鹅细小病毒(GPV)的全基因序列,针对两种病毒非结构蛋白基因序列的相同区段,分别设计了两对PCR引物LHMP/LHMP2和LHGP1/LHGP2,用这两对引物分别对MDPV和GPV进行检测,其结果引物LHMP1/LHMP2只特异性地扩增MDPV,而引物LHGP1/LHGP2也只特异性地扩增GPV,并且两对引物均扩增出约720bp的长度序列。
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关 键 词: | 番鸭 细小病毒 鹅 鉴别诊断 PCR技术 |
修稿时间: | 2000-02-14 |
Differential_Diagnosis of Muscovy Duck and Goose Parvoviruses using Polymerase Chain Reaction |
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Abstract: | By comparing complete nucleotide sequences ofMuscovy duck and Goose parvoviruses registered in the gene bank,two pairs ofprimers(LHMP1/LHGP2和LHGP1/LHGP2)were designed respectively corresponding to the sameregion of non_structural protein gene of the two viruses.MDPV and GPV were detected byusing these two primers.The first primer could only specifically amplify DNA of MDPV whichwere isolated from different areas;and the second primer also could only amplify that ofGPV.However,an 720_bp band all amplified in both cases. |
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