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| 毛花猕猴桃果实L-半乳糖内酯脱氢酶基因cDNA 片段的克隆与序列分析 | |
| 引用本文： | 燕标,吴延军,谢鸣,叶爱华.毛花猕猴桃果实L-半乳糖内酯脱氢酶基因cDNA 片段的克隆与序列分析[J].浙江农业学报,2011,23(3). |
| 作者姓名： | 燕标 吴延军 谢鸣 叶爱华 |
| 作者单位： | 1. 安徽农业大学,生命科学学院,安徽合肥230036;浙江省农业科学院,园艺研究所,浙江杭州310021 2. 浙江省农业科学院,园艺研究所,浙江杭州310021 3. 安徽农业大学,生命科学学院,安徽合肥230036 |
| 基金项目： | 浙江省科技厅面上项目，浙江省自然科学基金，宁波市农业成果转化项目，浙江省农业科学院院选项目 |
| 摘 要： | 毛花猕猴桃果实中Vc含量极高,为了揭示毛花猕猴桃Vc形成规律,根据已知植物的L-半乳糖内酯脱氢酶(GalLDH)基因,设计简并引物,以毛花猕猴桃新品种‘华特'果实为材料提取总RNA,采用RT-PCR法,克隆出一条长661 bp的cDNA片段,根据测序结果推测其氨基酸序列,并与刺梨、拟南芥、甘薯和草莓等植物的GalLDH基因编码的氨基酸序列进行亲缘分析.结果表明,所克隆片段编码氨基酸序列与美味猕猴桃GalLDH相似度高达99%,其在同源基因进化关系上聚为一类,与葡萄的GalLDH相似性高达91%,与草莓、刺梨、甘薯、温州蜜柑、拟南芥、甜瓜等植物的GalLDH同源性均在80%以上,可知克隆到的核苷酸片段确为毛花猕猴桃GalLDH基因的cDNA片段. |
| 关 键 词： | 毛花猕猴桃 L-半乳糖内酯脱氢酶 抗坏血酸 基因克隆 |
Cloning and sequence analysis of cDNA fragments encoding L-galactono-1,4-1actone dehydrogenase from fruit of Actinidia eriantha |
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| YAN Biao,WU Yan-jun,XIE Ming,YE Ai-hua.Cloning and sequence analysis of cDNA fragments encoding L-galactono-1,4-1actone dehydrogenase from fruit of Actinidia eriantha[J].Acta Agriculturae Zhejiangensis,2011,23(3). | |
| Authors: | YAN Biao WU Yan-jun XIE Ming YE Ai-hua |
| Institution: | YAN Biao1,2,WU Yan-jun2,XIE Ming2,YE Ai-hua1(1College of Life Sciences,Anhui Agricultural University,Hefei 230036,China,2Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China) |
| Abstract: | In order to investigate the molecular mechanism of Vc accumulation,661 bp cDNA encoding L-galactono-1,4-lacton dehydrognease(GalLDH) fragment was cloned from fruit of Actinidia eriantha by the method of RT-PCR,and then translated into amino acid sequences.In GenBank,compared with Actinidia delicisa,the amino acid sequence of GalLDH gene sequence showed 99% identity with those of Actinidia delicisa and they were both classified in the same family.The amino acid sequence of GalLDH gene sequence showed 91% ide... |
| Keywords: | kiwifruit L-galactono-1 4-lacton dehydrognease(GalLDH) ascorbic acid(AsA) gene cloning |
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