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| 一种快速筛选产聚羟基烷酸细菌方法的建立 | |
| 引用本文： | 孙燕飞,王翀,程模香,雷勇辉,熊杰.一种快速筛选产聚羟基烷酸细菌方法的建立[J].河南农业科学,2011,40(4):87-89,137. |
| 作者姓名： | 孙燕飞 王翀 程模香 雷勇辉 熊杰 |
| 作者单位： | 1. 新疆石河子大学生命科学学院,新疆石河子832003;新疆兵团绿洲生态农业重点实验室,新疆石河子832003 2. 新疆出入境检验检疫局,新疆乌鲁木齐,830063 3. 新疆兵团绿洲生态农业重点实验室,新疆石河子832003;新疆石河子大学农学院,新疆石河子832003 |
| 基金项目： | 石河子大学科学技术研究发展计划项目、高层次人才科研启动资金专项 |
| 摘 要： | 为了从土壤微生物中快速分离纯化得到产聚β-羟基脂肪酸(PHAs)的微生物菌株,研究比较了几种聚羟基烷酸产生菌筛选方法.结果表明,尼罗蓝染色法优于苏丹黑染色法,而且0.1%的尼罗蓝丙酮染液55℃染色细菌菌落10min,紫外灯下观察菌落,能合成聚羟基烷酸的菌落具有明显的荧光现象.进而推出对于产生物降解塑料菌株的染色,尼罗蓝... |
| 关 键 词： | 菌种筛选 聚羟基烷酸 尼罗蓝 苏丹黑 |
A Novel System for Selection of Polyester Biosynthetic Bacteria |
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| SUN Yan-fei,WANG Chong,CHENG Mo-xiang,LEI Yong-hui,XIONG Jie.A Novel System for Selection of Polyester Biosynthetic Bacteria[J].Journal of Henan Agricultural Sciences,2011,40(4):87-89,137. | |
| Authors: | SUN Yan-fei WANG Chong CHENG Mo-xiang LEI Yong-hui XIONG Jie |
| Institution: | 1,2(1.College of Life & Science,Shihezi University,Shihezi 832003,China;2.Key Laboratory of Oasis EcologyAgriculture of Xinjiang Bingtuan,Shihezi,Xinjiang 832003,China;3.Department of Agronomy Science,Shihezi University,Shihezi 832003,China;4.Xinjiang Exit-entry Inspection and Quarantine Bureau,Urumqi 830063,China) |
| Abstract: | In order to establish a simple,efficient and reliable method for selecting bacterium strains synthesizing polyhydroxyalkanoic acids(PHAs),several screening methods were compared.The results showed that the method of Nile blue dyeing was better than Sudan black dyeing.When colonies were dyed by Nile blue dissolved in acetone at 0.1% at 55℃ for 10min,and then visualized under UV(365nm),the positive colonies gave fluorescence.Therefore,Nile blue staining method was a better way compared to other screening methods. |
| Keywords: | Balteria screening Polyhydroxybutyrate(PHAs) Nile blue Sudan black |
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