| 培养环境条件对猕猴桃花粉萌发的影响 |
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| 引用本文： | 齐秀娟,张绍铃,方金豹.培养环境条件对猕猴桃花粉萌发的影响[J].浙江农业学报,2011,23(3):0. |
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| 作者姓名： | 齐秀娟 张绍铃 方金豹 |
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| 作者单位： | 1.中国农业科学院 郑州果树研究所,果树生长发育与品质控制重点开放实验室 河南 郑州 450009；2南京农业大学 园艺学院,江苏 南京 210095 |
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| 基金项目： | 基本科研业务费专项资助 |
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| 摘 要： | 采用离体培养法,在筛选出最佳培养基后,研究猕猴桃花粉在不同培养时间、pH值、培养基种类、温度条件下花粉萌发和花粉管伸长状况。结果表明：猕猴桃花粉离体萌发及花粉管伸长的最适培养基为10％蔗糖＋100 mg·L-1硼酸＋10 mg·L-1 Ca(NO3)2；离体培养最适观察时间为5 h,最适培养温度为30℃,最佳培养pH值为6.0。
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| 关 键 词： | 猕猴桃 培养条件 花粉萌发率 花粉管伸长 |
Effect of culture condition on pollen germination of kiwifruit |
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| QI Xiu-juan,ZHANG Shao-ling,FANG Jin-bao.Effect of culture condition on pollen germination of kiwifruit[J].Acta Agriculturae Zhejiangensis,2011,23(3):0. |
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| Authors: | QI Xiu-juan ZHANG Shao-ling FANG Jin-bao |
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| Institution: | 1Nanjing Agricultural University,Nanjing 210095, China；2Key Laboratory for Fruit Tree Growth, Development and Quality Control, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China |
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| Abstract: | The factors influencing pollen germination and tube length of kiwi in vitro were reported, including-different culture time, pH values, culture medium and culture temperature. The results showed that the most suitable culture medium for pollen germination and tube growth was 10％ sucrose+100 mg·L-1 boric acid+10 mg·L-1 Ca(NO3)2.The suitable culture time was 5 hours, the best temperature for pollen germination and growth was 30℃ and the suitable pH value of culture medium was 6.0. |
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| Keywords: | kiwifruit culture condition pollen germination pollen tube growth |
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