黄瓜花叶病毒甜菜分离物的分离鉴定

 从新疆石河子地区春季采种甜菜和夏播母根用甜菜上分离到3个病毒分离物,代号为石-B-2、石-B-5和石-B-D,自然感染甜菜引起叶片黄色花叶、扭曲、皱缩和植株严重矮化。病毒粒体球状、直径28~30 nm、均含有分子量约为2 9k D外壳蛋白、3种相同的较大片段的双链RNA (即3400 bp的RNA1、3100 bp的RNA₂、2300 bp的RNA₃)和片段大小约400 bp的小RNA,石-B-2和石-B-D具有1100 bp的RNA4,在ELISA和琼脂双扩散试验中,3个分离物均与CMV-83抗血清有特异反应,初步认为这3个球状病毒分离物属于CMV,但它们具有狭窄的寄主范围,不感染心叶烟、蔓陀罗、番茄,在寄主反应、双链RNA4和卫星RNA的大小上明显不同于国内外报道的CMV株系或分离物,同时说明侵染甜菜的CMV可能存在着株系分化。

ISOLATION AND IDENTIFICATION OF CUCUMBER MOSAIC VIRUS FROM SUGAR BEET

Three isolates (Shi B 2,Shi B 5 and Shi B D) were collected from naturally infected sugar beet with symptom of prominent yellow mosaic on severe distorted leaves and dwarfism in the region of Shihezi , Xinjiang . The virus particles all had isometric virons with diameters ranging from 28 30 nm, that with coat proteins estimated to be 29 kD, three large double strand RNAs of 3400 bp, 3100 bp, 2300 bp and a kind of smaller RNA about 400 bp. Isolates Shi B 2 and Shi B D contained an extra dsRNA that was 1100 bp. All the isolates positively reacted with antiserum of CMV 83 by agar double diffusion and ID ELISA, therefore the three isolates were preliminarily considered to be cucumber mosaic virus.However,they had narrower host range and could not infect N.glutinosa, Datura stramonium ,and tomato,the dsRNA 4 and satellite RNAs of the three isolates were also different from those in strains of CMV that havebeen reported so far, which indicate the strain differentiation among CMV infecting sugar beet.