| 茶树叶片抗寒相关蛋白提取方法比较研究 |
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| 引用本文： | 高永亮,王琴,陈林波,江昌俊. 茶树叶片抗寒相关蛋白提取方法比较研究[J]. 安徽农业大学学报, 2011, 38(3): 382-385. DOI: CNKI:34-1162/S.20110419.0925.021 |
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| 作者姓名： | 高永亮 王琴 陈林波 江昌俊 |
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| 作者单位： | 安徽农业大学茶叶生物化学与生物技术教育部重点实验室,合肥,230036;安徽农业大学茶叶生物化学与生物技术教育部重点实验室,合肥,230036;云南省农业科学院茶叶研究所,勐海,666201 |
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| 基金项目： | 国家自然科学基金(30871568); 安徽省自然科学基金(090411014)共同资助 |
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| 摘 要： | 对茶树叶片采用3种蛋白质提取方法,并对SDS-PAGE电泳结果进行了比较分析。结果表明,发现利用改良丙酮沉降法提取蛋白,不仅提取效率高,而且杂质干扰少。电泳结果显示,蛋白条带清晰,数量多。利用这一方法对冷驯化下不同品种茶树叶片抗寒相关蛋白进行了研究,在云抗43和舒茶早中分别发现了与抗寒相关的23 ku下调蛋白和51 ku上调蛋白,获得了满意的蛋白SDS-PAGE分析结果。
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| 关 键 词： | 茶树 蛋白提取 电泳 抗寒 |
Sifting of cold resistance protein from leaves of tea plants |
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| GAO Yong-liang,WANG Qing,CHEN Lin-bo and JIANG Chang-jun. Sifting of cold resistance protein from leaves of tea plants[J]. Journal of Anhui Agricultural University, 2011, 38(3): 382-385. DOI: CNKI:34-1162/S.20110419.0925.021 |
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| Authors: | GAO Yong-liang WANG Qing CHEN Lin-bo JIANG Chang-jun |
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| Affiliation: | GAO Yong-liang1,WANG Qing1,CHEN Lin-bo1,2,JIANG Chang-jun1(1.Key Laboratory of Tea Biochemistry & Biotechnology,Ministry of Education,Anhui Agricultural University,Hefei 230036,2.Tea Research Institute,Yunnan Academy of Agricultural Science,Menghai 666201) |
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| Abstract: | Three methods for extraction protein from tea plants were used,and the results were compared by SDS-PAGE.After optimization,satisfied results were achieved in the application of the modified acetone settle-ment method to isolate the cold resistance protein from several tea plants.23 ku down-regulation protein band in Yunkang43 and 51 ku up-regulation protein band in Shuchazao related to cold resistance could be observed in the analysis of SDS-PAGE. |
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| Keywords: | tea plant protein extraction electrophoresis cold resistance |
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