| TLC法测定几种植物中熊果酸的含量 |
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| 引用本文： | 于华忠,李国章,曹庸,龙维珍.TLC法测定几种植物中熊果酸的含量[J].西部林业科学,2005,34(1):60-61. |
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| 作者姓名： | 于华忠 李国章 曹庸 龙维珍 |
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| 作者单位： | 1. 湖南省林产化工工程重点实验室,湖南,张家界吉首大学,427000
2. 湖南省张家界林业科学研究所,湖南,张家界,427000 |
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| 摘 要： | 采用薄层扫描法对车前草、夏枯草、山茱萸中熊果酸的含量进行了测定, 其TLC测定条件为用环己烷-氯仿-乙酸乙酯(20 5 8) 作展开剂; 用10%H2SO4乙酸溶液作显色剂。测定结果表明: 3种植物中, 以夏枯草的熊果酸含量最高, 达0 812 %, 车前草的熊果酸含量最低, 为0 516 %, 前者是后者的1 57倍。该方法简便、快速、准确、可靠, 回收率高达96 1 %。
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| 关 键 词： | 薄层扫描法 熊果酸 含量测定 |
| 文章编号： | 1672-8246(2005)01-0060-02 |
| 修稿时间： | 2004年9月20日 |
The Determination of Ursolic Acid Content in Some Plants by the Method of thin-layer chromatography |
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| YU Hua-zhong,LI Guo-zhang,CAO Yong,LONG Wei-zhen.The Determination of Ursolic Acid Content in Some Plants by the Method of thin-layer chromatography[J].Journal of West China Forestry Science,2005,34(1):60-61. |
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| Authors: | YU Hua-zhong LI Guo-zhang CAO Yong LONG Wei-zhen |
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| Institution: | YU Hua-zhong~ |
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| Abstract: | The Ursolic acid content in Plantago major,Prunella asiatica and Cornus officinalis were determined by the method of thin-layer chromatography(TLC). The conditions of the determination by the method of TLC were as follows: The eluant is the mixture of cyclohexane, chloroform and acetic acid ethyl ester in the proportion of 20:5:8, and the color-developing agent is a 10% solution of hydric sulphate in acetic acid .The result showed that the Ursolic acid Content in Prunella asiatica is 0.812%, which is the highest in three plants, and that in Plantago major is 0.516%, the lowest in three plants. The former is 1.57 tims as much as the latter. This method is simple, fast, accurate and trustworthy, and its recovery ratio is 96.1%, which is very high. |
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| Keywords: | thin-layer chromatography ursolic acid the determination of the content |
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