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| 利用多重PCR技术鉴别牛早期胚胎性别研究 | |
| 引用本文： | 王昆,王栋,李魁英,张峰,马书林,吴占军,张新同,陈景顺. 利用多重PCR技术鉴别牛早期胚胎性别研究[J]. 中国奶牛, 2008, 0(4): 25-27 |
| 作者姓名： | 王昆 王栋 李魁英 张峰 马书林 吴占军 张新同 陈景顺 |
| 作者单位： | 1. 河北省农林科学院奶牛研究中心,石家庄,050031 2. 中国农业科学院畜牧研究所,北京,100094 3. 河北省农林科学院,石家庄,050051 |
| 基金项目： | 河北省科技攻关项目 , 河北省博士奖金项目 , 河北省财政人才基金 |
| 摘 要： | 本研究利用一对根据牛Y-染色体特异重复序列设计的牛Y-染色体特异性引物和一对根据牛微卫星DNA设计的牛常染色体内标引物，通过对PCR反应体系中Mg^2＋浓度和PCR反应条件中退火温度的优化，建立了牛早期胚胎性别鉴定的多重PCR反应体系。 |
| 关 键 词： | 性别鉴定 多重PCR 胚胎 |
| 文章编号： | 1004-4264(2008)04-0025-02 |
| 修稿时间： | 2007-03-01 |
Study on Sex Determination of Bovine Pre-implantation Embryos by Multi-PCR |
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| Wang Kun,Wang Dong,Li Kuiying,Zhang Feng,Ma Shulin,Wu Zhanjun,Zhang Xintong,Chen Jingshun. Study on Sex Determination of Bovine Pre-implantation Embryos by Multi-PCR[J]. China Dairy Cattle, 2008, 0(4): 25-27 | |
| Authors: | Wang Kun Wang Dong Li Kuiying Zhang Feng Ma Shulin Wu Zhanjun Zhang Xintong Chen Jingshun |
| Affiliation: | Wang Kun, Wang Dong, Li Kuiying, Zhang Feng, Ma Shulin, Wu Zhanjun, Zhang Xintong, Chen Jingshun (1.Dairy Cattle Research Center, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050031; 2.Institute of Animal Science, Chinese Academy of Agriculture Science, Beijing 100094) |
| Abstract: | Basing on the sequence of bovine Y-specific repeat sequence, a pair of bovine male-specific PCR primers were designed, meanwhile a pair of bovine-specific PCR primers for a sequence common to both sex were designed as internal control primers. Multi-PCR Conditions were optimized by varying the concentration of Mg^2＋, and the annealing temperature. Method of Multi-PCR for sexing Bovine preimplantation embryos was obtained. |
| Keywords: | Sex identification Muhi-PCR Embryos |
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