Functional Characterization of Citrus Polygalacturonase-inhibiting Protein |
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| Authors: | Sarunya NALUMPANG Yukie GOTOH Hiroyuki TSUBOI Kenji GOMI Hiroyuki YAMAMOTO Kazuya AKIMITSU |
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| Affiliation: | (1) United Graduate School of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan, JP;(2) Laboratory of Plant Pathology, Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan, JP;(3) Dainippon Ink and Chemicals, Incorporated, Agrichemicals Division, Central Research Laboratories, Sakura 285-8668, Japan, JP |
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| Abstract: | A cDNA encoding a polygalacturonase-inhibiting protein gene (SaiPGIPA) was identified from the citrus cultivar Sainumphung (Citrus sp.), one of the most popular cultivars in northern Thailand. SaiPGIPA was expressed in Escherichia coli cells, and the functional properties of citrus PGIP were analyzed. The PGIP fusion protein inhibited by a maximum of about 60% of the endopolygalacturonase activity, and a mixture of the PGIP and fungal endopoly-galacturonase released oligogalacturonides from polygalacturonic acid. The mixture containing the oligogalactur-onides, endopolygalacturonase and PGIP induced expression of the PGIP gene and a chalcone synthase gene in citrus leaves. The mixture also induced resistance in cucumber leaves against Colletotrichum lagenarium. Received 5 September 2001/ Accepted in revised form 20 November 2001 |
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| Keywords: | : polygalacturonase-inhibiting protein citrus Alternaria alternate polygalacturonase Sainumphung rough lemon. |
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