| 杨梅花色苷提取工艺条件的响应面分析及其抗氧化活性 |
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| 引用本文： | 戚向阳,姚叶斌.杨梅花色苷提取工艺条件的响应面分析及其抗氧化活性[J].果树学报,2009,26(2). |
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| 作者姓名： | 戚向阳 姚叶斌 |
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| 作者单位： | 戚向阳,QI Xiang-yang(浙江万里学院生物与环境学院,浙江宁波,315000;华中农业大学食品科技学院,武汉,430070);姚叶斌,YAO Ye-bin(浙江万里学院生物与环境学院,浙江宁波,315000)
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| 摘 要： | 为提高杨梅资源的利用和活性成分的开发,运用四元二次回归旋转组合设计法及分光光度法探讨了杨梅鲜果花色苷的最佳提取工艺及杨梅花色苷粗提物清除DPPH自由基的效果。结果表明,曲面回归方程与实验结果拟合性好,优化工艺为提取温度35.34℃,液料比(v/w)11.02∶1,乙醇体积分数为68.09%,pH值2.72。在该条件下,杨梅花色苷的提取率为94.33%。将杨梅花色苷提取液通过大孔树脂进行分离,所制备的杨梅花色苷粗提物其清除DPPH自由基的效果优于EGCG,其IC50为0.1026mg·L-1。研究结果表明杨梅粗提物具有较强的抗氧化活性,其中花色苷等酚类物质可能为杨梅中重要的抗氧化成分。
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| 关 键 词： | 杨梅 黄酮 提取工艺 优化 抗氧化 |
Response surface analysis of extraction technology for Chinese bayberry anthocyanins and their antioxidant activities |
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| QI Xiang-yang,YAO Ye-bin.Response surface analysis of extraction technology for Chinese bayberry anthocyanins and their antioxidant activities[J].Journal of Fruit Science,2009,26(2). |
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| Authors: | QI Xiang-yang YAO Ye-bin |
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| Institution: | 1Faculty of Bioscience and Environmental Science;Zhejiang Wanli University;Ningbo;Zhejian 315100 China;2College of Food Science and Technology;Huazhong Agricultural University;Wuhan;Hubei 430070 China |
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| Abstract: | In order to make full use of Chinese bayberry resource and exploit its active components,the optimal extraction technology for Chinese bayberry anthocyanins was studied by means of orthogonal rotational combination design,and the DPPH radical scavenging activity of the crude extract from fruits of Chinese bayberry was also researched.The results showed that regression equation fitted well with the experimental data,the optimum conditions of anthocyanins extraction were:ethanol concentration 68.09%,extractio... |
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| Keywords: | Chinese bayberry Anthocyanins Extraction technology Optimization Antioxidant activity |
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