| 大豆根系分泌物的鉴定及PAL1,PAL2,C4H的克隆 |
| |
| 引用本文： | 马凤鸣,王安娜,吴蕾,王婵婵,李业成,刘成,马国巍,石晓艳. 大豆根系分泌物的鉴定及PAL1,PAL2,C4H的克隆[J]. 作物杂志, 2011, 0(2): 65-71 |
| |
| 作者姓名： | 马凤鸣 王安娜 吴蕾 王婵婵 李业成 刘成 马国巍 石晓艳 |
| |
| 作者单位： | 东北农业大学农学院,150030,黑龙江哈尔滨 |
| |
| 基金项目： | 黑龙江省自然科学基金重点资助项目 |
| |
| 摘 要： | 为研究大豆根系分泌物在大豆连作障碍中的作用,利用土壤溶液原位取样器收集已灭菌的大豆正茬、一年重茬和两年重茬土壤盆栽的大豆根系分泌物,并采用GC-MS进行定性分析,检测出正茬、一年重茬和两年重茬的大豆根系分泌物,分别有355、7和64种,均含有烃、醇、酸、酯、酚、苯和醛等化合物。将收集的正茬、一年重茬和两年重茬的大豆根系分泌物溶液浇灌大豆幼苗,发现对茎长、茎鲜重、根长、根鲜重、光合速率和叶绿素含量均有一定的抑制作用。同时,以二氯甲烷(CH2Cl2)为溶剂,提取培养28d的大豆根系分泌物,共分离鉴定出大豆根系分泌物77种,其中包括烷烃、醇、酸、酯、苯、酚、萘、酰胺、酮、醛类以及其他类化合物。其中酚类物质占0.03%。基于追溯苯酚和苯甲酸类物质代谢的关键酶,克隆了苯丙氨酸解氨酶PAL家族的两个成员PAL1和PAL2,以及苯丙氨酸代谢的第二个关键酶C4H基因,为后续的基因操作提供依据。
|
| 关 键 词： | 大豆 根系分泌物 GC-MS 苯丙氨酸解氨酶 C4H |
Identification of Soybean Root Exudates and Cloning of the PAL1, PAL2, C4HGenes |
| |
| Ma Fengming,Wang Anna,Wu Lei,Wang Chanchan,Li Yecheng,Liu Cheng,Ma Guowei,Shi Xiaoyan. Identification of Soybean Root Exudates and Cloning of the PAL1, PAL2, C4HGenes[J]. Crops, 2011, 0(2): 65-71 |
| |
| Authors: | Ma Fengming Wang Anna Wu Lei Wang Chanchan Li Yecheng Liu Cheng Ma Guowei Shi Xiaoyan |
| |
| Affiliation: | Ma Fengming,Wang Anna,Wu Lei,Wang Chanchan,Li Yecheng,Liu Cheng,Ma Guowei,Shi Xiaoyan(College of Agriculture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China) |
| |
| Abstract: | Effect of the soybean root exudates on the soybean continuous cropping obstacles was studied.The soybean root exudates of the first stubble and the continuous cropping for one year and two years which were potted with the sterilized soil were extracted by the soil liquor sample collector in situ,and were isolated and identified by GC-MS qualitative analysis,which showed that the composition in the soybean root exudates of first stubble and continuous cropping for one year and two years were 35,57 and 64 spe... |
| |
| Keywords: | Soybean Root exudates GC-MS PAL C4H |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
