杨树Genomic-SSR与EST-SSR分子标记遗传差异性分析

利用16个杨树无性系对Genomic-SSR和EST-SSR两种SSR标记的遗传差异性进行分析。统计分析结果表明,Genomic-SSR平均检测到的等位基因数为4.1、Shannon指数为1.0646、观测杂合度为0.4427、期望杂合度为0.5523;EST-SSR平均检测到的等位基因数为2.8、Shannon指数为0.6985、观测杂合度为0.2330、期望杂合度为0.4684。聚类分析结果显示,EST-SSR相对Genomic-SSR能更精准地鉴别基因型。研究结果表明,在标记多态性及基因型鉴别等方面EST-SSR与Genomic-SSR均具有显著的遗传差异性。通过对两种分子标记遗传差异的比较分析,可为合理利用SSR标记进行物种遗传多样性等相关研究提供依据。

Genetic differences revealed by Genomic-SSR and EST-SSR in poplar

Sixteen poplar clones were used to analyse the genetic differences between Genomic-SSR and EST-SSR. The statistical results showed that the average number of alleles detected by Genomic-SSR was 4.1, Shannon was 1.064 6, heterozygosity was 0.442 7, expected heterozygosity was 0.552 3; while for the EST-SSR, the average number of alleles was 2.8, Shannon was 0.698 5, heterozygosity was 0.233 0, expected heterozygosity was 0.468 4. Cluster analysis indicated that the EST-SSR capacity of genotypes identification was more precise than Genomic-SSR. In a word, all these results reveal that the EST-SSR and Genomic-SSR have significant genetic differences in markers polymorphism and genotype identification, and that the differences would provide theoretical basis for the rational use of SSR marker in species diversity and other related research.