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| 海洋多粘类芽孢杆菌L1-9抗菌活性产物的分离与结构鉴定 | |
| 引用本文： | 马桂珍,付泓润,吴少杰,暴增海,王淑芳,葛平华.海洋多粘类芽孢杆菌L1-9抗菌活性产物的分离与结构鉴定[J].植物病理学报,2014,44(5):486-496. |
| 作者姓名： | 马桂珍 付泓润 吴少杰 暴增海 王淑芳 葛平华 |
| 作者单位： | 淮海工学院海洋学院,连云港 222005; 中国矿业大学化工学院,徐州 221116 |
| 基金项目： | 江苏省高校优势学科建设工程项目;江苏省农业科技支撑项目(BE2012375);江苏省高校自然科学重大项目(12KJA210001);连云港市科技局农业攻关项目(CN1207) |
| 摘 要： | 为了从海洋多粘类芽孢杆菌发酵液中分离鉴定抗菌活性产物,以对禾谷镰刀菌(Fusarium graminearum)的生物活性进行跟踪,采用硅胶柱层析、Sephadex LH-20柱层析、薄层层析和HPLC等技术,对海洋多粘类芽孢杆菌(Paenibacillus polymyxa)L1-9抗菌活性组分进行分离纯化,采用ESI-MS、1H NMR、13C NMR等波谱分析技术,对活性组分进行结构鉴定。采用牛津杯法测定活性组分的抑菌谱。得到了2种具有强抗菌作用的组分B1和B2;认为组分B1和B2分别为邻苯二甲酸二丁酯(DBP)和邻苯二甲酸二(2-乙基己基)酯(DEHP)。DBP和DEHP对供试的5种病原真菌和细菌具有抑制作用。邻苯二甲酸二丁酯的抑制作用高于邻苯二甲酸二(2-乙基己基)酯。 |
| 关 键 词： | 海洋多粘类芽孢杆菌 抗菌物质 分离鉴定 邻苯二甲酸二丁酯 邻苯二甲酸二(2-乙基己基)酯 |
| 收稿时间： | 2013-06-21 |
Isolation and structure elucidation of antifungal metabolites from marine Paenibacillus polymyxa strain L1-9 |
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| MA Gui-zhen,FU Hong-run,WU Shao-jie,BAO Zeng-hai,WANG Shu-fang,GE Ping-hua.Isolation and structure elucidation of antifungal metabolites from marine Paenibacillus polymyxa strain L1-9[J].Acta Phytopathologica Sinica,2014,44(5):486-496. | |
| Authors: | MA Gui-zhen FU Hong-run WU Shao-jie BAO Zeng-hai WANG Shu-fang GE Ping-hua |
| Institution: | College of Marine Science, Huaihai Institute of Technology, Lianyungang 222005, China; School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou 221116, China |
| Abstract: | The antibacterial metabolites from fermentation broth of marine Paenibacillus polymyxa strain L1-9 were purified by silica gel column chromatography, Sephadex LH-20, thin layer chromatography and HPLC methods, and the structures of these metabolites were identified by ESI-MS, 1H NMR、13C NMR and spectra analysis. The antibacterial activities were then determined by Oxford cup method. It was demonstrated that two kinds of compounds, B1 and B2 showed strong antibacterial activities, which were identified as dibutyl phthalate (DBP) and di(2-ethylhexyl)phthalate(DEHP), respectively. DBP and DEHP had inhibition effects on five kinds of fungi and bacteria. The inhibition effect of DBP was higher than that of DEHP. |
| Keywords: | marine Paenibacillus polymyxa antifungal compounds purification and identification dibutyl phthalate (DBP) di(2-ethylhexyl)phthalate(DEHP) |
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